THE EFFECT OF EXTRACTION METHODS ON BANDOTAN LEAF’S (Ageratum conyzoides L) ANTIBACTERIAL ACTIVITY
Keywords:
Bandotan leaves, maceration, soxhletation, antibacterialAbstract
Bandotan leaves (Argentum conizoydes L.) are often called nuisance plants. They grow wild in yards, plantation and fields. The chemical compound in bandotan leaves are potential as an antibacterial agent. The aims are to determine the effect of extraction method, by maceration and soxhletation, on the chemical compounds and antibacterial activity of bandotan leaf extract (Ageratum conizoydes L). Both are using 96% of ethanol. The phytochemical screening showed that both extracts contain alkaloids, flavonoids, tannins, saponins, and steroids, respectively. The photometric analysis showed the average concentration of flavonoids and tannins in bandotan leaf extract resulting from soxhletation, was 177,98 mg/g and 61,13 mg/g, higher than that of maceration, 133,67 mg/g and 47,75 mg/g. The antibacterial activity of extract from soxhletation had a greater inhibitory power 7.524 mm±1.457 compared to the extract from maceration 4.749 mm±2.142. It showed that the extraction method affected the concentration of chemical compounds and antibacterial activity.
References
Diniatik. (2015). Penentuan Kadar Flavonoid Total Ekstrak Etanolik Daun Kepel (Stelechocarpus Burahol (Bl.) Hook F. & Th.) Dengan Metode Spektrofotometri. Kartika-Jurnal Ilmiah Farmasi, Jun 2015, 3 (1), 1-5
Harborne J.B. 1987. Metode Fitokimia. Edisi ke-2. Padmawinata K, Soediro I, penerjemah. Bandung: Institut Teknologi Bandung. Terjemahan dari: Phytochemical Methods.
Irawan, H. (2014) ‘Pengaruh Konsentrasi Pelarut Etanol Terhadap Profil Kromatogram Dan Kandungan Senyawa Kimia Dalam Ekstrak Daun Pepaya ( Carica papaya L . ) Dan Daun Patikan Kebo (Euphorbia hirta L.)’, Article, pp. 40–45.
Kadji, M. H., M. R. J. Runtuwene., dan G. Citraningtyas. (2013). Uji Fitokimia dan Aktivitas Antioksidan dari Ekstrak Etanol Daun Soyogik (Saurauia bracteosa DC). FMIPA UNSRAT. Manado.
Laoli, N. S. 2018. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Bandotan (Ageratum conyzoides L.) Terhadap Bakteri Bacillus substilis dan Proteus vulgaris. Tugas Akhir. Program Studi Sarjana Farmasi Fakultas Farmasi Universitas Sumatera Utara Medan 4, pp. 67–73.
Rosita, J. M., Taufiqurrahman, I. and Edyson (2017) ‘Perbedaan Total Flavonoid antara Metode Maserasi dengan Sokletasi pada Ekstrak Daun Binjai (Mangifera caesia)’, Dentino Jurnal Kesdokteran Gigi, I(1), pp. 100–105.
Sulastri, Taty., (2009). Analisis Kadar Tanin Ekstrak Air dan Ekstrak Etanol pada Biji Pinang Sirih (Areca Catechu. L). Jurnal Chemica Vo/. 10 Nomor 1 Juni 2009, 59-63.
Sulistyarini, I., Sari, D. A. and Wicaksono, T. A. (2019) ‘Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus)’, Jurnal Ilmiah Cendekia Eksakta, pp. 56–62.
Wardaningrum, (2019) ‘Perbandingan Aktivitas Antioksidan Ekstrak Etanol Terpurifikasi Ubi Jalar Ungu (Ipomoea batata L) dengan Vitamin E’, artikel, 52(1), pp. 1–5.
x
Wipalangga, A. Sari, L.P. (2018). Analisis Fitokimia dan Antioksidan Metode DPPH Ekstrak Metanol Daun Salam (Eugenia polyantha). Ijobb, pp. 19-24.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 PHARMACON
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
