Uji Toksisitas Ekstrak Etanol Bunga Kamboja Kuning (Plumeria alba L.) dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT)
Keywords:
Bunga Kamboja Kuning (Plumeria alba L.), Senyawa Fitokimia, Sitotoksik, BSLT, LC50.Abstract
Indonesia memiliki keanekaragaman tanaman yang sangat berlimpah sehingga banyak masyarakat menggunakan tanaman sebagai pengobatan trasdisional, yaitu salah satunya tanaman kamboja. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas sitotoksik dari ekstrak etanol bunga kamboja kuning (Plumeria alba L.) terhadap larva udang Artemia salina Leach. Proses ekstraksi menggunakan pelarut etanol dan diuji secara kualitatif terhadap alkaloid, flavonoid, tanin, saponin dan steroid/triterpenoid. Uji sitotoksik menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan konsentrasi larutan uji 1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL, 62,5 µg/mL dan 31,25 µg/mL. Hasil uji fitokima menunjukkan bahwa sampel mengandung senyawa alkaloid, flavonoid, saponin, tanin maupun triterpenoid dan tidak mengandung senyawa steroid. Hasil uji sitotoksik menunjukkan bahwa sampel bersifat toksik dengan nilai LC50 301,38 µg/mL. Dapat disimpulkan bahwa sampel  mengandung senyawa alkaloid, flavonoid, saponin, tanin dan triterpenoid dan bersifat toksik terhadap larva udang Artemia Salina Leach sehingga dapat dikembangkan sebagai obat antikanker.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 PHARMACON
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
