Antibacterial Activity Test of African Leaf Extract (Vernonia amygdalina) Against Staphylococcus aureus and Pseudomonas aeruginosa Bacteria Growth.
Abstract
African leaves (Vernonia amygdalina) are known to be one part of the plant that has antibacterial activity that has the potential to be developed as a traditional medicine. This study aims to determine antibacterial activity, the effect of increasing extracts and the most effective concentration on the inhibitory power of the growth of Staphylococcus aureus and Pseudomonas aeruginosa bacteria. The antibacterial activity of african leaf ethanol extract with concentrations of 5%, 10%, 20%, 40%, 80% was tested using the disc diffusion method by observing the diameter of the inhibitory zone compared to Ciprofloxacin as a positive control and CMC as a negative control. The results of measurements of the diameter of the inhibition zone concentrations of 5% (7.0 mm), 10% (9.2 mm), 20% (11.5 mm), 40% (13.8 mm), 80% (15.7 mm) in S. aureus bacteria, 5% (8.1 mm), 10% (9.1 mm), 20% (11.6 mm), 40% (13.1 mm), 80% (15.1 mm) in P. aeruginosa bacteria have provided activity to inhibit the growth of test bacteria. The results were analyzed using One Way ANOVA and continued with the Duncan test. Research shows that the higher the concentration of the extract, the greater the diameter of the inhibitory zone of bacterial growth. An extract concentration of 80% is the most effective concentration for inhibiting S. aureus and P. aeruginosa bacteria.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 PHARMACON
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)