UJI AKTIVITAS ANTIBAKTERI EKSTRAK SPONS Callyspongia aerizusa DARI PERAIRAN DESA POOPOH KABUPATEN MINAHASA
DOI:
https://doi.org/10.35799/pha.13.2024.49230Keywords:
Callyspongia aerizusa, Antibakteri, Staphylococcus aureus, Pseudomonas aeruginosaAbstract
Callyspongia aerizusa is one of the sponge species that is spread across the Indonesian marine waters and rich in bioactive compounds such as secondary metabolites that is used in medicine field as antibacterial but still not widely used by the people. The improvement of bacteria resistant has been a serious problem in the medicine field, therefore there should be an improvement of a new antibiotic medicine from natural ingredients for the healt improvement. This research aims to find the activity of extracts of sponge Callyspongia aerizusa from the waters of Poopoh Village, Minahasa Regency against the growth of Pseudomonas aeruginosa and Staphylococcus aureus bacteria. The growth of bacterial is tested by disc diffusion Kirby Bauer method and by observing the value of generated inhibition zone. From the result of the research, the extracts of Callyspongia aerizusa sponge has clear zone against Staphylococcus aureus bacteria of 8 mm in the concentration of 250 µg/disc, and the Pseudomonas aeruginosa bacteria has 9 mm in the concentration of 250 µg/disc which categorized as medium. It can be concluded that the Callyspongia aerizusa sponge has an metabolite compound with wide spectrum activity that can hinder the growth of Gram-negative and Gram-positive bacteria
References
Ananto, F.J., Herwanto, E.S., Nugrahandhini, N.B., Najwa, Y.C., Abidin, M.Z., Suswati, I. 2015. Gel Daun Kelor Sebagai Antibiotik Alami Pada Pseudomonas Aeruginosa Secara In Vivo. Pharmacy. 12(1)
Anjaswati, D., Pratimasari, D., Nirwana, A.P. 2021. Perbandingan Rendemen Ekstrak Etanol, Fraksi nHeksana, Etil Asetat, dan Air Daun Bit (Beta vulgaris L.) Menggunakan Fraksinasi Bertingkat. Jurnal Farmasi (Journal of Pharmacy). 2(1)
Awaeh, E.L., Wewengkang, D.S., Lebang, J.S. 2022. Potensi Ekstrak Dan Fraksi Spons Callyspongia Aerizusa yang diperoleh Dari Perairan Pulau Manado Tua Terhadap Pertumbuhan Bakteri Staphylococcus Aureus dan Escherichia Coli. Pharmacon. 11(3).
Damayanti, A., Ilyas, A., Firnanelty. 2020. Senyawa Golongan Alkaloid dari Ekstrak Etanol Spons Stylotella sp. Asal Kepulauan Selayar. Alchemy: Journal Of Chemistry. 8(2): 12-15
De Sousa, L.H.N., De Araújo, R.D., Sousa- Fontoura, D., Menezes, F.G., Araújo, R.M. 2021. Metabolities from Marine Sponges of the Genus Callyspongia: Occurrence, Biological Activity, and NMR Data. Journal Marine Drugs. 19(12): 663.
Faiza, F. 2015. Isolasi Dan Karakterisasi Senyawa Alkaloid Spons Callyspongia Sp. Perairan Biak Papua. [Skripsi]. Bandar Lampung: Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Lampung.
Hafsari, A. R., Cahyanto, T., Sujarwo. T., Lestari,R. I. 2015. Uji Aktivitas Antibakteri Ekstrak Daun Beluntas (Pluchea Indica (L.) Less.) Terhadap Propionibacterium Acnes Penyebab Jerawat [Skripsi]. Bandung: Fakultas Sains Dan Teknologi, UIN Sunan Gunung Djati.
Herfianto, P.N., Nurhuda, M., Yuana, F. 2014. Pengaruh Durasi Evaporasi Etanol Low Grade Terhadap Kadar Etanol Pada Residu Hasil Evaporasi. Brawijaya Physics Student Journal. 1(1)
Herlina, N., Fifi, A., Aditia, D.C., Poppy, D.H., Qurotunnada., Baharuddin, T. 2015. Isolasi Dan Identifikasi Staphylococcus Aureus Dari Susu Mastitis Subklinis Di Tasikmalaya. Jawa Barat: Pros Sem Nas Masy Biodiv Indon. 1(3): 413-417.
Kowal, A., Esther, A., Nickson, K., Kurniati, K., Henky, M., Deiske, H. 2018. Potensi antibakteri karang lunak lobophytumsp. Dari perairan pangalisang pulau bunaken terhadap bakteri Pseudomonas aeruginosa dan Staphylococcus aureus. Jurnal Ilmiah Platax. 6(2).
Kusuma, A.H., Muhaemin, M., Mayagues, M., Efendi, E. 2023. Rehabilitasi Ekosistem Terumbu Karang Menggunakan Terumbu Buatan Di Perairan Desa Kunjir, Kecamatan Rajabasa, Kabupaten Lampung Selatan, Provinsi Lampung. Jurnal Pengabdian Fakultas Pertanian Universitas Lampung. 2(1): 280 – 293
Lalamentik, G. 2017. Aktivitas Antibakteri Ekstrak Karang Lunak Klyxum Sp. Yang Diperoleh Dari Teluk Manado. [Skripsi]. Manado: Program Studi Farmasi FMIPA Universitas Sam Ratulangi.
Leba, M.A.U. 2017. Buku Ajar: Ekstraksi dan Real Kromatografi. Cetakan Pertama. Yogyakarta: CV. Budi Utama
Mardiyah, U., Fasya, G.A., Fauziyah, B., Amalia, S. 2014. Ekstraksi Uji Aktivitas Antioksidan dan Identifikasi Golongan Senyawa Aktif Alga Merah Eucheuma spinosum dari Perairan Banyuwangi. Jurnal Achemy. 3(1) : 42
Marzuki, I. 2018. Eksplorasi Spons Indonesia Seputaran Kepulauan Spermonde. Makasar: Penerbit Nas Media Pustaka
Meldha., Wewengkang, D.S., Mansauda, K.L.R. 2021. Uji Aktivitas Antibakteri Ekstrak Dan Fraksi Spons Callyspongia Aerizusadari Perairan Pulau Mantehage Manado. Pharmacon. 10(3)
Mubarak., Fhahri., Sartini., Purnawati, D. 2018. Effect of Ethanol Concentration on Antibacterial Activity of Bligo Fruit Extract (Benincasa hispida Thunb) to Salmonella typhi. Indonesian Journal of Pharmaceutical Sciences and Technology. 5(3): 77
Nahor, E.M., Rumagit, B.I., Tou, H.Y. 2020. Perbandingan Rendemen Ekstrak Etanol Daun Andong (Cordyline futicosa L.) Menggunakan Metode Ekstraksi Maserasi dan Sokhletasi. Poltekkes Kemenkes Manado. 1(1): 40
Nurhaniefah, A.A., Sagitasa, S., Aulia, S., Hadisaputri, Y.E. Callyspongia sp.: Secondary Metabolites, Pharmacological Activities, and Its Mechanisms. Indonesian Journal of Biological Pharmacy. 2(2): 104-120
Oroh, S.B., Kandou, F.E.F., Pelealu, J., dan Pandiangan, D. 2015. Uji Daya Hambat Ekstrak Metanol Selaginella delicatula dan Diplazium dilatatum terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli. Jurnal Ilmiah Sains. 15(1): 52–58.
Ortez, J. H. 2005. Disk Diffusion testing in manual of antimicrobial susceptibility testing. Marie B. Coyle (Coord. Ed). American society for Microbiology. America.
Paju, N., Yamlean, P.V., Kojong, N. 2013. Uji Efektivitas Salep Ekstrak Daun Binahong (Anredera Cordifolia Steenis.) Pada Kelinci (Oryctolagus Cuniculus) Yang Terinfeksi Bakteri Staphylococcus Aureus. Pharmacon 2(1): 51–61.
Pujiastuti, N.I. 2022. Uji Aktivitas Antibakteri Pseudomonas Aeruginosa Dan Stabilitas Fisik Gel Ekstrak Daun Kelor (Moringa Oleifera) Dengan Metode Ultrasonik. [Skripsi]. Malang : Universitas Islam Negeri Maulana Malik Ibrahim
Putri, R.A., Simbala, H.E., Mpila, D.A. 2020. Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine americana Merr) Terhadap Bakteri Staphylococcus aureus, Escherichia coli dan Salmonella typhi. Pharmacon. 9(4): 525-532
Silap, G.E., Wewengkang, D.S., Rotinsulu. H. 2020. Uji Aktivitas Antimikroba Karang Lunak Dendronephtya Sp. yang Dikoleksi dari Desa Tumbak Kecamatan Pusomaen, Kabupaten Minahasa Tenggara terhadap Escherichia coli, Staphylococcus aureus, dan Candida albicans. Pharmacon. 9(1): 64-65.
Suriani, S., Soemarno., Soeharjono. 2013. Pengaruh Suhu dan pH terhadap Laju pertumbuhan Lima Isolat Bakteri Anggota Genus Pseudomonas yang diisolasi dari Ekosistem Sungai Tercemar Deterjen di sekitar Kampus Universitas Brawijaya. Jurnal Pembangungan dan Alam Lestari. 3(2): 1-5.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Viocindy Riska Nusaly, Defny S.Wewengkang, Erladys Rumondor
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)
