TOXICITY TESTING OF SOURSOP LEAF EXTRACT (Annona muricata l.) AS AN ANTICANCER CANDIDATE WITH THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD
DOI:
https://doi.org/10.35799/pha.13.2024.49449Keywords:
Toxicity, Anticancer, Annona muricata Linn, Brine Shrimp Lethality Test (BSLT), LC50 Value.Abstract
Soursop leaves (Annona muricata L.) contain several secondary metabolite compounds including alkaloids, flavonoids, tannins, saponins, steroids and is one of the plants used to prevent and treat cancer. This study was conducted with the aim of knowing whether the secondary metabolite compounds contained in soursop leaves and the potential of soursop leaves as anticancer candidates against Artemia salina Leach larvae. The extraction method used to extract soursop leaves is the maceration method using 96% ethanol solvent. Phytochemical screening tests in this study include examination tests for alkaloids, flavonoids, tannins, saponins, steroids and triterpenoids. Toxicity testing using the Brine Shrimp Lethality Test (BSLT) method using 4 concentrations (1000 ppm, 100 ppm, 10 ppm, 1 ppm) and carried out three replication. The results of research obtained in phytochemical screening tests, soursop leaves have alkaloid, flavonoids, tannins, saponins, and compound. The results showed that the total death toxicity testing at a concentration of 1000 ppm 83%, at a concentration of 100 ppm 67%, at a concentration of 10 ppm 47% and at a concentration of 1 ppm 30%. The higher the concentration of soursop leaf extract, the higher the mortality from shrimp larvae and the LC50 value of 12.57 ppm. Based on the LC50 value , it can be concluded that soursop leaf extract has the potential as an anticancer candidate and is very toxic because had an LC50 which < 30 ppm.
References
Alim Nur., dkk, 2021. Skirining Fitokimia Ekstrak Etanol Kulit Buah Sirsak (Annona muricata Linn) dan Uji Aktivitas Antioksidan dengan Metode DPPH. Sasambo Journal of Pharmacy. 2(2): 60-64.
Emi N Rosidah. 2019. Uji Toksisitas Ekstrak Labu Kuning (Cucurbita moschata Durch.) terhadap larva udang Aretmia salina dengan Metode BSLT (Brine Shrimp Lethality Test). [Skripsi]. UIN Sunan Ampel : Surabaya.
Friyan C. Manopo. 2021. Skrining Fitokimia Dan Uji Toksisitas Ekstrak Etanol Umbi Bawang Dayak (Eleutherine Sp.) Dengan Metode BSLT (Brine Shirmp Lethality Test). [Skripsi]. Manado : Universitas Sam Ratulangi.
Hadi Kurniawan., Meri Ropiqa. 2021. Uji Toksisitas Ekstrak Etanol Daun Ekor Kucing (Acalypha hispida Burm.f.) Dengan Metode Brine Shrimp Lethality Test (BSLT). Sciences and Clinicsl Research. 3(2): 52-62.
Halimah, N. 2010. Uji Fitokimia dan Uji Toksisitas Ekstrak Tanaman Anting-Anting (Acalypha indica L.) terhadap Larva Udang Artemia salina L. [Skripsi]. Fakultas Sains dan Teknologi, Universitas Islam Negeri Maulana Malik Ibrahim. Malang.
Hanifah, N. 2015. Uji Toksisitas Akut Ekstrak Metanol Daun Sirsak (Annona Muricata L) Terhadap Larva Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT). [Skripsi]. Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Syarif Hidayatullah. Jakarta.
I Putu Andika Arianta. 2021. Uji Toksisitas Ekstrak Etanol Bunga Kamboja Kuning (Plumeria alba) dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). [Skripsi]. Manado : Universitas Sam Ratulangi.
Kemenkes RI. 2018. Hasil Utama Riset Kesehatan Dasar 2018. Kementrian Kesehatan Badan Penelitian dan Pengembangan Kesehatan : Jakarta.
Laurencia Diva B. Artantyo. 2021. Skrining Fitokimia dan Uji Toksisitas Ekstrak Etanol Daun Miana Merah (Coleus hybridus) dengan Metode Brine Shrimp Lethality Test (BSLT). [Skripsi]. Manado : Universitas Sam Ratulangi.
Mardiana, L. 2011. Ramuan Dan Khasiat Daun Sirsak. Jakarta: Penebar Swadaya.
Mayang A., Santoso S. A. B., 2020. Uji Toksisitas Akut Infusa Daun Sirsak (Annona muricata L.) Pada Larva Artemia Salina Leach Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Pharmacy Medical Journal. 3(1):25.
Ningsih R. D., Zusfahair, Dwi Kartika. 2016. Identifikasi Senyawa Metabolit Sekunder Serta Uji Aktivitas Ekstrak Daun Sirsak Sebagai Antibakteri. Jurnal Ilmiah Kimia. 11(10).
Prayoga Eka G. D., dkk, 2019. Identifikasi Senyawa Fitokimia Dan Aktivitas Antioksidan Ekstrak Kasar Daun Pepe (Gymnema reticulatum Br.) Pada Berbagai Jenis Pelarut. Jurnal Ilmu dan Teknologi Pangan. 4(2):111-121.
Reskianigsih A. 2014. Uji Toksisitas Akut Ekstrak Metanol Buah Phaleria macrocarpa (Scheff) Boerl Terhadap Larva Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test (BSLT). [Skripsi]. Jakarta: Universitas Islam Negri Syarif Hidayattulah Jakarta.
Sulistyarini I., Sari D. A., Wicaksono T A. 2020. Skrining Fitokimia Senyawa Metabolit Sekunder Batang Buah Naga (Hylocereus polyrhizus). Jurnal Ilmiah Cendekia Eksakta. 5(1).
Sumiati Triyani, Effendi F., Puspitasari R A. 2016. Uji Toksisitas ekstrak Daun Sirsak (Annona muricata L.) Yang Berpotensi Sebagai Antikanker. Pharmamedika Journal. 1(2):85-91.
Surya, A. 2018. Toksisitas Ekstrak Metanol Kulit Jengkol (Pithecellobium Jiringa) dengan Metode Brine Shrimp Lethality Test terhadap Larva udang (Artemia salina). Jurnal Rekayasa Sistem Industri. 3(2):149-153.
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