Eco-Enzyme as a Bioactivator for Converting Organic Wastes into Organic Fertilizer: Effects on Chili Pepper (Capsicum Annuum L.) Growth and Yield in Tomohon, Indonesia.

Authors

  • Maria Goretti Meity Polii Sam Ratulangi University
  • Antje Grace Tulungen Sam Ratulangi University
  • Stanley A. F. Walingkas Sam Ratulangi University
  • Saartje Sompotan Sam Ratulangi University

DOI:

https://doi.org/10.35791/jat.v7i1.66742

Keywords:

Capsicum annuum, eco-enzyme, market waste, organic fertilizer, waste valorization

Abstract

Eco-enzyme has been promoted as a low-cost bioactivator for converting organic residues into useful soil amendments, but comparative agronomic evidence across different waste substrates remains limited. This study evaluated eco-enzyme–assisted organic fertilizers prepared from several locally available organic wastes and assessed their effects on chili pepper (Capsicum annuum L.) growth and yield in Kakaskasen, Tomohon City, North Sulawesi, Indonesia, from April to October 2025. A pot experiment was arranged in a completely randomized design with six treatments and four replications (24 experimental units). Eco-enzyme was produced by fermenting fruit peels, sugar, and water (3:1:10) for three months. Organic fertilizers were prepared using eco-enzyme alone or eco-enzyme combined with vegetable waste, fruit waste, market waste, chicken-manure waste, or household organic waste, and were incorporated into the pot medium one week before transplanting at an equivalent rate of 20 t ha⁻¹. Plant height, fruit number per plant, and fresh fruit weight per plant were measured at 100 days after transplanting and analyzed using analysis of variance followed by the least significant difference test at 5%. Organic-waste substrate significantly affected all measured variables. The eco-enzyme + market-waste formulation produced the tallest plants (133.37 cm), the highest fruit number (89.50 fruits plant⁻¹), and the highest mean fresh fruit weight (225.25 g plant⁻¹), markedly exceeding eco-enzyme alone (69.12 cm; 36.75 fruits plant⁻¹; 78.75 g plant⁻¹). Overall, eco-enzyme–activated market-waste fertilizer was the most effective formulation for improving chili growth and yield under the conditions of this pot experiment.

Keywords: Capsicum annuum; eco-enzyme; market waste; organic fertilizer; waste valorization

References

REFERENCES

Bang, D., Chung, W., & Chang, S. (2024). Influence of Effective Microbial Additives Inoculation on Indigenous Bacterial Community Dynamics and Co-Occurrence Patterns During the Composting of Mixed Food Waste and Livestock Manure. In Agronomy (Vol. 14, Issue 12, p. 2973). https://doi.org/10.3390/agronomy14122973

Benny, N., Shams, R., Dash, K. K., Pandey, V. K., & Bashir, O. (2023). Recent trends in utilization of citrus fruits in production of eco-enzyme. Journal of Agriculture and Food Research, 13, 100657. https://doi.org/https://doi.org/10.1016/j.jafr.2023.100657

Carpanez, T., Castro, L., Moreira, B., Carneiro, J., Gomes, E., Otenio, M., Silva, J., Balarini, J. C., Damasceno, F., Amaral, M., & Moreira, V. (2025). Soil fertilization potential and nutrient dynamics of organic fertilizers derived from sugarcane residues under techno-economic and environmental assessment. Chemosphere, 389, 144708. https://doi.org/10.1016/j.chemosphere.2025.144708

Djunaedy, A., Khoiri, S., Firdaus, N., Megasari, D., & Giyanto. (2024). Field trial of new Bt-base bioinsecticide formula, Bashield®, for controlling Spodoptera frugiperda J.E. Smith on maize. E3S Web of Conferences, 499. https://doi.org/10.1051/e3sconf/202449901031

Dubey, A., Gaur, H., Manzoor, U., Singh, S., Singh, P., Devi, C., Yadav, J., Choudhary, M., & Ahmed, B. (2025). Bioconversion of horticultural waste into value-added products: A Review. Indian Journal of Horticulture, 82, 375–394. https://doi.org/10.58993/ijh/2025.82.4.1

Faisal, A. F., & Mustafa, Y. F. (2025). Chili pepper: A delve into its nutritional values and roles in food-based therapy. Food Chemistry Advances, 6, 100928. https://doi.org/https://doi.org/10.1016/j.focha.2025.100928

Filogônio, I., Bringhenti, J., Pires, P., & Korres, A. (2023). The use of commercial inoculant and efficient microorganisms (EM) in small-scale composting. OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, 21, 16311–16340. https://doi.org/10.55905/oelv21n10-099

Gasperoni, F., Ieva, F., & Paganoni, A. (2025). ANOVA: Analysis of Variance (pp. 227–263). https://doi.org/10.1007/978-3-031-86670-8_11

Jeong, Y.-J., Vyavahare, G. D., Kim, S.-H., Jeon, S.-H., Roh, A.-S., Yun, J.-J., & Shim, J.-H. (2025). Effect of fertilizer and food waste compost on soil carbon, nitrogen use efficiency, and yield of Chinese cabbage (Brassica Rapa L.). Applied Biological Chemistry, 68(1), 77. https://doi.org/10.1186/s13765-025-01044-3

Jones, G. P., Stambaugh, C., Stambaugh, N., & Huber, K. E. (2023). Chapter 30 - Analysis of variance. In A. E. M. Eltorai, J. A. Bakal, D. W. Kim, & D. E. B. T.-T. R. O. Wazer (Eds.), Handbook for Designing and Conducting Clinical and Translational Research (pp. 171–177). Academic Press. https://doi.org/https://doi.org/10.1016/B978-0-323-88423-5.00041-8

Madan, S., Narang, N., Hussain, A., & Priyadarshi, M. (2025). Exploring the impact of eco-enzyme diversity on the physicochemical characteristics of rice husk and sawdust compost. Environmental Monitoring and Assessment, 197(4), 348. https://doi.org/10.1007/s10661-025-13793-1

Mahardika, I., Wirajaya, A., & Arjana, I. (2025). Application of Organic Fertilizer from Rabbit and Cattle Farm Waste on the Growth and Yield of Chili peppers (Capsicum annuum L.). AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment), 9, 200–205. https://doi.org/10.29165/ajarcde.v9i1.548

Narang, N., Hussain, A., & Madan, S. (2024). A comparative study on compost preparation using lab prepared eco-enzyme and its effect on growth of plant species Phaseolus vulgaris. Environmental Science and Pollution Research, 31(25), 36230–36240. https://doi.org/10.1007/s11356-023-27168-x

Pasalari, H., Moosavi, A., Kermani, M., Sharifi, R., & Farzadkia, M. (2024). A systematic review on garbage enzymes and their applications in environmental processes. Ecotoxicology and Environmental Safety, 277, 116369. https://doi.org/https://doi.org/10.1016/j.ecoenv.2024.116369

Ramamoorthy, K., Dhanraj, R., Vijayakumar, N., Ma, Y., Al Obaid, S., & Narayanan, M. (2024). Vegetable and fruit wastes: Valuable source for organic fertilizer for effective growth of short-term crops: Solanum lycopersicum and Capsicum annum. Environmental Research, 251(Pt 2), 118727. https://doi.org/10.1016/j.envres.2024.118727

Retnowati, D., Kumoro, A., & Haryani, K. (2023). Anaerobic Fermentation of Mixed Fruits Peel Waste for Functional Enzymes Production Employing Palm Sugar and Molasses as The Carbon Source. Reaktor, 23, 71–76. https://doi.org/10.14710/reaktor.23.2.71-76

Soni, S. K., & Soni, R. (2025). Nutrient Recovery and Soil Enrichment: Sustainable Practices for Resource Recycling (pp. 209–248). https://doi.org/10.1007/978-981-96-9013-8_7

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Published

2025-12-31

How to Cite

Polii, M. G. M., Tulungen, A. G., Walingkas, S. A. F., & Sompotan, S. (2025). Eco-Enzyme as a Bioactivator for Converting Organic Wastes into Organic Fertilizer: Effects on Chili Pepper (Capsicum Annuum L.) Growth and Yield in Tomohon, Indonesia. Jurnal Agroekoteknologi Terapan (JAT), 7(1), 177–184. https://doi.org/10.35791/jat.v7i1.66742

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