Efectiveness Of Plant Preservative Mixture (Ppm) Biocides on Contamination Levels in In-Vitro Propagation of Chrysanthemum (Chrysanthemum morifolium) Plants.

Mario Paul Stevanus Weken; Jeany Polii Mandang; Edy Fredy Lengkong

doi:10.35791/jat.v7i2.67929

Authors

Mario Paul Stevanus Weken Sam Ratulangi University. Indonesia
Jeany Polii Mandang Sam Ratulangi University. Indonesia
Edy Fredy Lengkong Sam Ratulangi University. Indonesia

DOI:

https://doi.org/10.35791/jat.v7i2.67929

Keywords:

Chrysanthemum, Contamination, In-vitro, Biocide Plant Preservative Mixture (PPM), Sterilization

Abstract

This study aims to determine the interaction of the use of explant sterilization with Plant Preservative Mixture (PPM) biocides and the optimal dose of this biocide. This research was conducted at the Plant Biotechnology Laboratory, Faculty of Agriculture, Sam Ratulangi University, Manado. This study used a factorial Completely Randomized Design (CRD) which consisted of two treatment factors, namely explant sterilization (P) and PPM dose (Q) so that there were 8 treatment combinations. The variables observed were: the percentage of fungal contamination, the percentage of bacterial contamination, the percentage of media contamination, the percentage of explant contamination, the percentage of explant death and the day the first contamination appeared.The results showed that the best treatment combination was P2Q4 (Explants Sterilization + PPM 2.5 ml/l). In addition, the explant sterilization treatment and the use of PPM biocide had an interaction with total contamination, fungal contamination, bacterial contamination, media contamination and the day the first contamination appeared, but had no interaction with explant contamination and explant death.

Keywords: Chrysanthemum, Contamination, In-vitro, Biocide Plant Preservative Mixture (PPM), Sterilization.

Abstrak. Tujuan penelitian ini untuk mengetahui interaksi sterilisasi eksplan menggunakan biosida Plant Preservative Mixture (PPM) beserta dosis optimal penggunaan biosida ini. Penelitian ini dilaksanakan di Laboratorium Bioteknologi Tanaman, Fakultas Pertanian, Universitas Sam Ratulangi Manado. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari dua faktor perlakuan yaitu sterilisasi eksplan (P) dan dosis PPM (Q) sehingga terdapat 8 kombinasi perlakuan. Variabel yang diamati adalah: Persentase kontaminasi jamur, persentase kontaminasi bakteri, persentase kontaminasi media, persentase kontaminasi eksplan, persentase kematian eksplan dan hari muncul kontaminasi pertama. Hasil penelitian menunjukan kombinasi perlakuan terbaik yaitu terletak pada perlakuan P2Q4 (Sterilisasi Eksplan + PPM 2.5 ml/l). Selain itu, faktor perlakuan sterilisasi eksplan dan penggunaan biosida PPM memiliki interaksi terhadap variabel total kontaminasi, kontaminasi jamur, kontaminasi bakteri, kontaminasi media dan hari muncul kontaminasi pertama, tetapi tidak memiliki interaksi terhadap variabel kontaminasi eksplan dan kematian eksplan.

Kata kunci: Krisan, Kontaminasi, In-vitro, Biosida Plant Preservative Mixture (PPM), Sterilisasi.

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