AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Lobophytum sp. TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

Authors

  • Queen N.H. Loing

DOI:

https://doi.org/10.35799/pha.5.2016.11231

Abstract

AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Lobophytum sp. TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

Queen Novika Hermina Loing1), Defny Silvia Wewengkang1), Jemmy Abidjulu 1)

1)Program Studi Farmasi FMIPA UNSRAT Manado

 

 

ABSTRACT

Indonesia is a tropical country which is have so many abundant biodiversity, and one of them is soft coral. This research aims to test the antibacterial activity of soft coral Lobophytum sp. fractions against the Escherichia coli and Staphylococcus aureus. Extraction was done by maceration and fractionation using n-hexane, chloroform, methanol and water as a solvent. The test of antibacterial activity was done by using agar diffusion method. The result shows that extract and fractions have an antibacterial activity. The results conclude that the extract and fractions of Lobophytum sp. effectively inhibit the Escherichia coli and Staphylococcus aureus, in medium and strong category. The Strong inhibitory capacity showed by chloroform fraction.

Key words : Lobophytum sp, antibacterial activity, Escherichia coli, Staphylococcus aureus, fractionation, agar diffusion.

ABSTRAK

Indonesia merupakan negara tropis, yang mempunyai keanekaragaman hayati berlimpah salah satunya ialah karang lunak. Penelitian ini bertujuan untuk menguji aktivitas antibakteri fraksi karang lunak Lobophytum sp. terhadap bakteri Escherichia coli dan Stapylococcus aureus. Ekstraksi menggunakan metode maserasi dengan pelarut etanol dan fraksinasi menggunakan pelarut n-heksan, kloroform, metanol dan air. Pengujian antibakteri mengunakan metode difusi agar. Hasil penelitian menunjukkan ekstrak dan fraksi uji memiliki aktivitas sebagai antibakteri. Berdasarkan hasil penelitian, disimpulkan bahwa ekstrak dan fraksi karang lunak Lobophytum sp efektif dalam menghambat pertumbuhan bakteri Escherichia coli serta bakteri Staphylococus aureus dengan kategori sedang dan kuat. Daya hambat terbesar ditunjukkan oleh fraksi kloroform.

 

Kata kunci : Lobophytum sp, antibakteri, Escherichia coli, Staphylococcus aureus, fraksinasi, difusi agar.

 

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Published

2016-02-09

How to Cite

Loing, Q. N. (2016). AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Lobophytum sp. TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus. PHARMACON, 5(1). https://doi.org/10.35799/pha.5.2016.11231

Issue

Vol. 5 No. 1 (2016): Pharmacon

Section

Articles

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