Screening of the Proteolytic Bacteria Symbiont with Algae Gracillaria sp.

Riorifki Kabense, Elvy L. Ginting, Stenly Wullur, Nickson J. Kawung, Fitje Losung, Jhon L. Tombokan

Abstract


Marine algae are abundant natural resources in Indonesia but have not been optimally utilized. Utilization of seaweed is still limited as food ingredients such as Gracillaria sp. cultivated as an industrial export material. Algae Gracillaria sp. his life is symbiotic with a variety of types of bacteria. The aim of the study was to isolate and screening the protease activity of the symbionic bacteria of Gracillaria sp. This study succeeded in isolating 4 different bacteria based on morphological characteristics. The four isolates were S.G.,1,  S.G.2, S.G.3 and S.G.,4. Isolate S.G. 1 had the ability to produce ptotease with a proteolytic index of 1.5.

Keywords : Gracilaria sp., Protease, Symbiont Bacteria

 

ABSTRAK

Alga laut merupakan sumberdaya alam yang melimpah di Indonesia tetapi belum optimal dimanfaatkan oleh masyarakat. Pemanfaatan rumput laut masih terbatas sebagai bahan makanan seperti Gracillaria sp. dibudidayakan sebagai bahan ekspor industri karajinan. Alga Gracillaria sp. hidupnya bersimbion dengan beraneka ragam jenis bakteri. Tujuan penelitian adalah untuk mengisolasi dan menguji aktivitas protease bakteri simbion alga Gracillaria sp. Penelitian ini berhasil mengisolasi 4 bakteri yang berbeda berdasarkan karakteristik morfologi. Keempat bekteri tersebut adalah S.G., 1 S.G., 2 S.G., 3 dan S.G., 4 Isolat bakteri  S.G., 1 memiliki kemampuan   menghasilkan ptotease dengan Indeks proteolitik sebesar 1,5.

Kata Kunci  : Bakteri simbion, Gracillaria sp. protease


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DOI: https://doi.org/10.35800/jip.7.2.2019.24487

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