ISOLASI DAN IDENTIFIKASI SENYAWA FLAVONOID DALAM DAUN BELUNTAS (Pluchea indica L.)

Abstract

Isolation and identification flavonoid compounds in beluntas leaf (Pluchea indica L.) have been
conducted. Isolation was carried out by using 96% p.a ethanol solvent of maceration extraction for 7
days and partition using n-heksana solvent to obtain concentrated beluntas leaf extract. The extract
was purified by thin layer chromatography using eluent n-butanol : asetic acid : water (BAA) (4:1:5).
Isolate 3, result from thin layer chromatography contain flavonoid compound. Ultra Violet - Visible
spectra showed that the flavonoid compound was flavonol, with characteristic wavelengths from 250
to 280 nm for band II and 350-385 nm for band I.
Keywords: beluntas, flavonoid, Thin Layer Chromatography, UV-Vis Spectrophotometer