ISOLASI DAN IDENTIFIKASI FLAVONOID DALAM DAUN LAMUN (SYRINGODIUM ISOETIFOLIUM)

Abstract

Sea grass is a group of flowering plant, leaved, has its own roots and live in a shallow water.
It usually used to capture sediment, compost, and as antioxidant. The use of sea grass on
human needs to be conducted. Because of its potency and its component such as flavonoids, a
research to identify kind of flavonoids in sea grass must be conducted. The aims of this
research are to isolate and identify flavonoids in sea grass (Syringodium isoetifolium) using
thin layer chromatography and spectrophotometer UV-Vis. Extraction was done with
maceration using ethanol 96% as solvent. Isolation was done by Thin Layer Chromatography
GF 6054, and n-butanol : acetic acid : water (BAA) (4:1:5). There are two spot after isolate
with TLC, the first is yellow spot with Rf 0,4 and second is red with Rf 0,8. Identification of
flavonoids was done using spectrophotometer UV-Vis. Based on results, sea grass consist on
flavonoids, suspected chalcon group.
Keywords : sea grass, flavonoids, thin layer chromatography, UV-vis