Antibacterial Activity of Secondary Metabolits Isolated from Mangrove Leaves Sonneratia alba
DOI:
https://doi.org/10.35799/jis.v22i2.42332Keywords:
antibacterial activity, secondary metabolites, Sonneratia albaAbstract
Mangroves Sonneratia alb is a plants that grows in tidal areas with considerable environmental pressure. This condition causes the plant to synthesize secondary metabolites that have certain bioactivity to protect it self from destruction. The purpose of this study was to isolate antibacterial secondary metabolites from the mangrove leaves of  S.alba. The method applied was to isolate secondary metabolites from the ethyl setate fraction using column chromatography and identified the results of isolation with thin layer chromatography until a pure isolate was obtained. Pure isolates were characterized in structure using HNMR, IR and LC-MS spectroscopy. The resulting isolate in the form of methanol-soluble oil had the molecular formula C24H38O4 with the name Di(2-ethylhexyl)phthalate. The antibacterial activity of this compound was the same as the antibacterial activity of amoxicillin as a positive control, namely the diameter of the inhibition zone was 7 mm at a sample concentration of 0,21%.
Aktivitas Antibakteri Metabolit Sekunder yang Diisolasi dari Daun Mangrove Sonneratia alba
ABSTRAK
Mangrove termasuk Sonneratia alba adalah tumbuhan yang tumbuh pada daerah pasang surut dengan tekanan lingkungan yang cukup besar, keadaan ini menyebabkan tumbuhan mangrove menyintesis metabolit sekunder yang mempunyai bioaktivitas tertentu untuk melindungi diri dari perusakan. Tujuan dari penelitian ini adalah mengisolasi metabolit sekunder antibakteri dari daun mangrove  S.alba.. Metode yang digunakan adalah mengisolasi metabolit sekunder pada fraksi etil asetat menggunakan kromatografi kolom dan identifikasi hasil isolasi dengan kromatografi lapis tipis hingga didapatkan isolat murni. Isolat murni dikarakterisasi strukturnya menggunakan spektroskopi HNMR, IR dan LC-MS. Isolat yang dihasilkan berupa minyak yang larut dalam metanol mempunyai rumus molekul C24H38O4 dengan nama Di(2-etilhexil)fthalat. Aktivitas antibakteri dari senyawa ini  sama dengan aktivitas antibakteri amoxicilin sebagai kontrol positif yaitu diameter zona hambat sebesar 7 mm pada konsentrasi sampel 0,21%.
Kata kunci: Aktivitas antibakteri; metabolit sekunder; Sonneratia alba
References
Adhikary, A., Ray, M., Das, A.K., & Sur, T. K. 2018. Antidiabetic and Antioxidant Activity of Rhyzophora mucronata Leaves (Indian Sundarban Mangrove): An in Vitro and In Vivo Study. International Quarterly Journal of Research , 37(1): 76-81.
Cahyadi, J., Satriani, G.I., Gusman, E., & Sabri. 2018. Skrining Fitokimia Ekstrak Buah mangrove (Sonneratia alba) Sebagai Bioenrichment Pakan alami Artemia Salina. Phytochemical Jurnal Borneo Saintek, 1(3): 33-39.
Damongilala, L.J., Losung, F., & Dotulong, V. 2021. Aktivitas Antibakteri Ekstrak Rumput Laut Eucheuma spinosum Segar dari Perairan Pulau Nain Sulawesi Utara. Jurnal Ilmiah Sains, 21(1): 91-95.
Darminto, A., & Dini, I. 2012. Isolasi Senyawa Metabolit Sekunder Utama Ekstrak Etanol Kulit Batang Tumbuhan Mangrove (Avicennia spp). Jurnal Sainsmart, 1(1): 61-67.
Das, S.K., Samantaray, D., Sahoo, S.K., Pradhan, S.K., Samanta, Samanta, L., 2019. Bioactivity Guidet Isolation of Antidiabetic and Antioxidant Compount from Xylocarpus granatum J Koenic bark. Biotech, 9(5): 1-9.
Dotulong, A.R., Dotulong, V., Wonggo, D., Montolalu, L.A., Harikedua, S.D., Mentang, F. 2020. Metabolit Sekunder Ekstrak air Mendidih Daun Mangrove Sonneratia alba. Media Teknologi Hasil Perikanan , 8(2): 66-69.
Dotulong, V. 2014. Aktifitas Antioksidan Alga Laut Caulerpa sertularoides, Laurencia tronoi, Padina australis, Isolasi dan Elusidasi Senyawa Aktif pada Fraksi n-Heksana Laurencia tronoi. Disertasi. Universitas Brawijaya, Program Studi Ilmu Pertanian. Universitas Brawijaya, Malang.
Dotulong, V., Wonggo, D., & Montolalu, L. A. 2018. Phytochemical Content, Total Phenols and Antioxidant Activity of Mangrove Sonneratia alba Young Leaf Through Extraction Methods and Solvents. International Journal of ChemTech , 11(11): 356-363.
Elfita, Munawar, & Wahyuni, S. 2014. Isolation of Secondary Metabolite Compounds of Phatalate Derivatives from Endophytic Fungi of Brotowali Plant (Tinospora crispa L). Biodevercsity Seminar Proeeiding, (pp. 2(3): 241-244.
Garg, B., Bihst, T., & Ling, Y.C. 2014. Sulfonated Graphene as Highly Efficient and Reusable Acid Carbocatalyst for The Synthesis of Ester Plasticizers Electronic Suplementary Material (ESI) for RSC Advances . The Royal Society of Chemistry , 4(100): 1-15.
Harizon, Pudjiastuti, B., Kurnia, D., Samiarsa, D., & Shiono. 2014. Triterpenoid Lupan dari Kulit Batang Sonneratia alba (Lytraeceae) . Bionatura-Jurnal Ilmu-Ilmu Hayati dan Fisik , 16(1): 25-29.
Herawati, N. 2011. Identifikasi Senyawa Bioaktif Tumbuhan Mangrove Sonnerati alba. Jurnal Chemical, 12 (2): 54-58.
Karim, M.A., Islam, A., Islam, M.M., Rahman, M. S., Sultana, S., Biswas, S. 2020. Evaluation of Antioxidant , Anti Hemolytic, Cytocxic Effects and Antibacterial of Selected Mangrove Plants (Bruguera gymnorrhiza and Heritiera litoralis in Bangladesh Activity. Clinical Phytiscience, 6(8): 1-12.
Latief, M., Nelson, Amanda, H., Tarigan, I. L., & Aisyah, S. 2020. Potential Trackingof Citotoxic Activities of Mangrove Perepate (Sonneratia alba) Root Extract as an Anti-cancer Candidate. Pharmacology and Clinical Pharmacy Reseach, 6(8):1-12.
Muharni, Elfita, & Masyita. 2015. Isolasi Senyawa Metabolit Sekunder dari Ekstrak n-Heksana Batang Tumbuhan Brotowali (Tinospora crispa). Molekul, 10(1): 38-44.
Musa, W.J., Duengo, S., & Situmeang, B. 2018. Isolatin and Characterization TYriterpenoid Compound from Leaf Mangrove Plant (Sonneratia alba) and Antibacterial Activity Tes. Int. Res. J. Pharm, 9(3): 85-89.
Noor, Y.R., Khazali, M., & Suryadipura, I.N. 2012. Panduan Pengenalan Mangrove Indonesia.
Nurmalasari, F., Ersam, T., & Fatimawati, S. 2016. Isolasi senyawa Antioksidan dari kulit Batang Sonneratia Ovata Backer. Jurnal sains dan seni, 5(2): 149-153.
Palaniayandi, T., Sivaji, A., Thiruganasambandam, R., Natarajan, S., & Hari, R. 2018. In Vitro Antigastric Cancer Activity of Squalene, a Triterpenoid Compound Isolated from Rhizophora Mucronata Mangrove Plant Leaves Againts AGS Cell Line. Pharmacognosy Magizine, 14(57): 369-375.
Schaduw, J.N. 2016. Kondisi Ekologi Mangrove Pulau Bunaken Kota Manado Provinsi Sulawesi Utara. Jurnal LPPM Bidang Sains dan Teknologi , 3(2): 64-74.
Shobi, T.M., & Viswathan, M.B. 2018. Anztibacterial Activity of Di-butyl Phatalate Isolated from Begonia malabaric. Journal of Applied Biotechnology dan Bioengineering, 5(2): 97-100.
Stephen, J. (2005). Manual of Antimicrobial Susceptibility Testing. American Society fore Microbiology.
Usman, Amir, M., Erika, F., Nurdin, M., & Kuncoro, H. 2019. Antidiabetic Activity of Leaf Extract from Three Types of Mangrove Originating from Sambera Coastal Region Indonesia. Reseach Journal Pharm and Tech, 12(4): 1707-1712.
Wonggo, D., Berhimpon, S., Kurnia, D., & Dotulong, V. 2017. Antioxidan Activity of Sonneratia alba Fruit from Wori Vilage Nort Sulawesi Indonesia. International Journal of ChemTech Research, 10(12): 284-290.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Verly Dotulong, Lena Jeane Damongilala
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
LICENCE: CC-BY-NC
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
