PENGEMBANGAN PRODUK SEDIAAN GEL KOMBINASI EKSTRAK DAUN SIRSAK (Annona muricita L.) DENGAN EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorhiza Roxb.) SEBAGAI ANTI BAKTERI PENYEBAB JERAWAT (Propionibacterium acne dan Staphylococcus epidermidis)
DOI:
https://doi.org/10.35799/pha.6.2017.17973Abstract
PENGEMBANGAN PRODUK SEDIAAN GEL KOMBINASI EKSTRAK DAUN SIRSAK (Annona muricita L.) DENGAN EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorhiza Roxb.) SEBAGAI ANTI BAKTERI PENYEBAB JERAWAT (Propionibacterium acne dan Staphylococcus epidermidis)
Â
Christel Nataniel Sambou1) , Agung Eru Wibowo1) , Shelly Taurhesia1)
1)Program Studi Magister Ilmu Kefarmasian Universitas Pancasila Jakarta, 12640
Â
ABSTRACT
One of the causes of acne is the presence of Propionibacterium acnes and Staphylococcus epidermidis on skin. The aim of this research was to develop gel product made from the combination of extracts that are effective as anti-acne against P. Acnes and S. epidermidis. This research was based on experiment method. Soursop leaf and temulawak rhizome were extracted by maceration in ethanol 96%. The viscous extract was then tested to P.Acnes and S.epidermidis to gain Minimum Inhibitory Concentration (MIC). The MIC value of soursop leaf and temulawak rhizome extract for P. Acnes and S.epidermidis were 3% b/v and 1% b/v respectively. The antibacterial potential of formulated gel was gained by evaluating the average diameter of the radical zone with three repetitions of gel contained ethanol extract of temulawak rhizome 1% b/v against bacteria P. acnes and S. epidermis (1,33 mm and 1,67 mm), ethanol extract of soursop leaf 3% b/v (1,33 mm and 1,67 mm), combination of temulawak rhizome and soursop leaf 3:1% b/v (2 mm and 2,67 mm), 1,5:1% b/v (1,67 mm and 1,63 mm), and 4,5:1% b/v (3,67 mm and 3,63 mm). The result showed that in gels contained the combination of of temulawak rhizome and soursop leaf with the similar concentration of single extract did not have the better radical zone result compared to the single extracts, and were considered as antagonist.
Keywords : Annona muricita L., Curcuma xanthoriza Roxb., Â Propionibacterium acnes, Staphylococcus epidermidis
ABSTRAK
Salah satu penyebab munculnya jerawat adalah berkembangnya bakteri Propionibacterium acne dan Staphylococcus epidermidis. Penelitian ini bertujuan untuk mengembangkan produk sediaan gel dari kombinasi ekstrak yang  efektif sebagai anti jerawat terhadap bakteri P. Acnes dan S.epidermidis. Penelitian ini menggunakan metode eksperimen. Daun sirsak dan rimpang temulawak di ekstraksi menggunakan metode maserasi dengan pelarut etanol 96%. Ekstrak kental yang diperoleh kemudian dilakukan uji anti bakteri P. Acnes dan S.epidermidis untuk mendapatkan nilai Konsentrasi Hambat Minimum (KHM). Nilai KHM ekstrak daun sirsak dan ekstrak rimpang temulawak untuk bakteri P. Acnes dan S.epidermidis berturut-turut adalah 3% b/v dan 1% b/v. Hasil uji anti bakteri sediaan di peroleh  diameter rataan zona bunuh/zona bening bakteri (radical zone) dengan 3 kali pengulangan untuk Gel ekstrak etanol rimpang temulawak konsentrasi 1% b/v pada bakteri P. Acnes dan S.epidermidis (3,33 mm dan 2,67 mm), ekstrak etanol daun sirsak konsentrasi 3% b/v (1,33 mm dan 1,67 mm), kombinasi konsentrasi ekstrak rimpang temulawak dan ekstrak daun sirsak 3:1% b/v (2 mm dan 2,67 mm), kombinasi konsentrasi 1,5:1% b/v (1,67 mm dan 1,63 mm), kombinasi konsentrasi 4,5:1% b/v (3,67 mm dan 3,63 mm). Hasil uji tersebut bersifat antagonis karena pada gel kombinasi kedua ekstrak dengan konsentrasi yang sama dengan ekstrak tunggal tidak memberikan zona bunuh yang lebih baik dari sediaan gel ekstrak tunggalnya.
Kata kunci : Daun sirsak, rimpang temulawak, Propionibacterium acne, Staphylococcus epidermidis
Â
Â
Â
Â
Â
Â
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)