AKTIVITAS ANTIBAKTERI FRAKSI TERIPANG LAUT Holothuria atra TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus

Authors

  • Pasia M.T. Umboh

DOI:

https://doi.org/10.35799/pha.7.2018.21429

Abstract

AKTIVITAS ANTIBAKTERI FRAKSI TERIPANG LAUT
Holothuria atra TERHADAP BAKTERI Escherichia
coli DAN Staphylococcus aureus
Pasia MitraTryhilami Umboh1), Defny Silvia Wewengkang1), Paulina V. Y Yamlean1)
1)Program Studi Farmasi FMIPA UNSRAT Manado, 95115
ABSTRAK
Indonesia merupakan negara tropis, yang mempunyai keanekaragaman hayati berlimpah salah
satunya ialah teripang laut. Penelitian ini bertujuan untuk menguji aktivitas antibakteri fraksi teripang laut
(Holothuria atra) terhadap bakteri Escherichia coli dan Staphylococcus aureus. Ekstraksi menggunakan
metode maserasi dengan pelarut etanol dan fraksinasi menggunakan pelarut n-heksan, kloroform, metanol
dan air. Pengujian antibakteri menggunakan metode difusi agar. Hasil penelitian menunjukkan ekstrak
dan fraksi uji memiliki aktivitas sebagai antibakteri.Berdasarkan hasil penelitian, disimpulkan bahwa
fraksi teripang laut (Holothuria atra) efektif dalam menghambat pertumbuhan bakteri Escherichia coli
serta bakteri Staphylococcus aureus dengan kategori sedang dan kuat.Daya hambat terbesar ditunjukkan
oleh fraksi kloroform.
Kata Kunci : Holothuria atra, daya hambat, Escherichia coli, Staphylococcus aureus,
fraksinasi, difusi agar.
ABSTRACT
Indonesia is a tropical country, which has abundant biodiversity and one of them is sea
cucumber. This study aims to examine the antibacterial activity of sea cucumber (Holothuria atra)
fraction against Escherichia coli and Staphylococcus aureus bacteria. Extraction uses a maceration
method with ethanol and fractionation using solvent of n-hexane, chloroform, methanol and water
solvents. Antibacterial testing using agar diffusion method. The results showed that the extract and
fraction of the test had activity as antibacterial. Based on the results of the study, it was concluded that
the fraction of sea cucumber (Holothuria atra) was effective in inhibiting the growth of Escherichia coli
bacteria and Staphylococcus aureus bacteria with medium and strong category. The largest inhibitory
factor is shown by the chloroform fraction.
Keywords : Holothuria atra, inhibitory factor, Escherichia coli, Staphylococcus aureus,
fractionation, agar diffusion.

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Published

2018-10-26

How to Cite

Umboh, P. M. (2018). AKTIVITAS ANTIBAKTERI FRAKSI TERIPANG LAUT Holothuria atra TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus. PHARMACON, 7(4). https://doi.org/10.35799/pha.7.2018.21429

Issue

Vol. 7 No. 4 (2018): Pharmacon

Section

Articles

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