EVALUASI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL SPONS Aplysina sp. DARI PERAIRAN SELAT LEMBEH KOTA BITUNG

Authors

  • Aprilia Manumpil Program Studi Farmasi, Fakultas MIPA, Universitas Sam Ratulangi
  • Defny S Wewengkang Program Studi Farmasi, Fakultas MIPA, Universitas Sam Ratulangi
  • Adithya Yudistira Program Studi Farmasi, Fakultas MIPA, Universitas Sam Ratulangi

DOI:

https://doi.org/10.35799/pha.8.2019.29246

Abstract

ABSTRACT

Aplysina sp. Sponge is one of the marine biota making up coral reefs that contain active compounds whose percentage of activity is greater than the compounds produced by land plants. This study aims to determine the activity of antioxidant compounds from the ethanol extract of Aplysina sp. from Lembeh Strait Waters, Bitung City. Sponge Aplysina sp., was extracted by maceration with ethanol as a solvent. As a parameter, testing of antioxidant activity was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, which was measured using UV-Vis Spectrophotometry at a wavelength of 517 nm. The result showed the ethanol extract of Aplysina sp., sponge proven to have antioxidant activity in each concentration test. The highest concentration has an antidote to free radical activity by reaching a percentage of 46,13%.

 

Keywords: Aplysina sp. Sponge, Antioxidant, Extraction, DPPH

 

ABSTRAK

Spons Aplysina sp. merupakan salah satu biota laut penyusun terumbu karang yang mengandung senyawa aktif yang presentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol Spons Aplysina sp. dari Perairan Selat Lembeh, Kota Bitung. Spons Aplysina sp. diekstraksi menggunakan metode maserasi dengan etanol sebagai pelarut. Sebagai parameter, pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil) yang diukur menggunakan Spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil penelitian menunjukkan ekstrak etanol spons Aplysina sp. terbukti memiliki aktivitas antioksidan disetiap konsentrasi pengujian.Konsentrasi tertinggi memiliki aktivitas penangkal radikal bebas dengan mencapai presentase 46,13%.

 

Kata Kunci : Spons Aplysina sp, Antioksidan, Ekstraksi, DPPH

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Published

2019-02-28

How to Cite

Manumpil, A., Wewengkang, D. S., & Yudistira, A. (2019). EVALUASI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL SPONS Aplysina sp. DARI PERAIRAN SELAT LEMBEH KOTA BITUNG. PHARMACON, 8(1), 127–133. https://doi.org/10.35799/pha.8.2019.29246

Issue

Vol. 8 No. 1 (2019): PHARMACON

Section

Articles

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