Uji Aktivitas Antioksidan Ekstrak Etanol Karang Lunak Lobophytum sp. yang diperoleh dari Pantai Tongkaina, Kota Manado

Chika Wanda Sumare; Adithya Yudistira; Yuanita Amalia Hariyanto

doi:10.35799/pha.15.2026.63998

Authors

Chika Wanda Sumare Universitas Sam Ratulangi
Adithya Yudistira Universitas Sam Ratulangi
Yuanita Amalia Hariyanto Universitas Sam Ratulangi

DOI:

https://doi.org/10.35799/pha.15.2026.63998

Keywords:

Karang lunak, Fitokimia, Ekstrak Etanol, Pantai Tongkaina, Kota Manado, Bahan Alam, Aktivitas Biologis, Senyawa Bioaktif

Abstract

Soft coral contain bioactive compounds, such as alkaloids, alkaloids, flavonoids, and saponins, which have important antioxidant activity. This research aims to tested the antioxidant activity of ethanol extract of Lobophytum sp. using method DPPH (2,2-diphenyl-1-picrylhydrazyl). Phytochemical screening results show the presence of tannins, flavonoids, and saponins in the extract of Lobophytum sp. obtained from Tongkaina Beach, Manado City. Antioxidant activity test shows that the extract has high activity an IC₅₀ value of 49,13 ppm which is categorized as very strong. Average value of percent inhibition increased along with increasing extract concetration, reaching 67,13% at concetration of 100 ppm. These findings indicate that Lobophytum sp. potential be a source of natural antioxidants that can be developed for prevention degenerative disease caused by free radicals. Further research necessary to explore more specific active compounds.

Keywords: Antioxidant, IC₅₀, Lobophytum sp., Percent inhibition, Phytochemical Screening

References

Aderiyanti, R. 2022. Studi Perbandingan Metode Pengkuran Antioksidan. Skripsi. Universitas Islam Negeri Raden Intan Lampung.

Apri, R., Neviaty Zamani, Hefni Effendi. 2013. Eksplorasi Karang Lunak Sebagai Antioksidan di Pulau Pongkok Bangka Selatan. Jurnal Teknologi Perikanan dan Kelautan. 4(2): 211-217.

Blois, M.S. 1958. "Antioxidant determinations by the use of a stable free radical." Nature. 181: 1199-1200.

Doe, J., Smith, A., & Johnson, R. 2023. Antioxidant properties of flavonoids, alkaloids, and saponins: A review. Journal of Medicinal Plant Research, 11(5), 45-60.

Harris, D. C. 2010. Quantitative Chemical Analysis. 8th Edition. W.H. Freeman and Company.

Kadar, A. 2015. Pengelolaan Kemaritiman Menuju Indonesia Sebagai Poros Maritim Dunia. Jurnal Keamanan Nasional. Lembaga Concern (Consultancy and Research) 3(1): 427.

Macias, F. A., & Varela, R. M. 2007. Extraction of alkaloids from plants. In Techniques in the Analysis of Alkaloids. Springer.

Molyneux, P. 2018. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin Journal Science Technolgy. 26(2): 211-219.

Nur, R. M., Mu'nisa, A., & Hala, Y. 2019. Skrining Fitokimia Ekstrak Metanol Karang Lunak Lobophytum sp. Jurnal Bionature. 20(1): 57-63.

Parwata, M. O. A. 2016. Antioksidan. Kimia Terapan Program Pascasarjana Universitas Udayana. 1–54.

Radjasa O.K., D. S. Kencana, A. Sabdono, R.A.Hutagalung and E.S. Lestari. 2007. Antibacterial Activity of Marine Bacteria Associated with sponge Aaptos sp. against Multi Drugs Resistant (MDR) strains. Jurnal Matematika Dan Sains. 2(4):147-152.

Risna M. Nur Mu’nisa, A., Hala, Y. 2019. Skrining Fitokimia Ekstrak Metanol Karang Lunak Lobophytum sp. Jurnal Bionature. 20(1), 57-63.

Rumondor, E. M., Adithya Yudistira., Wewengkang, D. S., South, E. J., Rotinsulu., Tumondo, B. T., & Kindangen, E. E. 2024. Penentuan nilai IC50 Karang Lunak Lobophytum sp. dan Sarcophyton sp. dari Perairan Pantai Parentek Kabupaten Minahasa. Pharmacy Medical Journal.

Suryanti, T., & Iskandar, S. 2019. Screening of bioactive compounds from marine sponges of the genus Callyspongia and their potential in pharmaceutical applications. Journal of Marine Drugs, 17(12), 1-15.

Tristantini, D., Ismawati, A., Pradana, B. T., & Jonathan, J. G. (2016). Pengujian aktivitas antioksidan menggunakan metode DPPH pada daun tanjung (Mimusops elengi L). In Seminar Nasional Teknik Kimia" Kejuangan" (p. 1)

Wanda E, Sadarun B. 2018. Keanekaragaman dan Kepadatan Karang Lunak di Perairan Waworaha Kecamatan Soropia. Sapa Laut. 3(1), 9-15.

Wink, M. 2010. Alkaloids: Biochemistry, Ecology, and Medicinal Applications. Springer.

Yulianto, I., & Supriharyono, I. 2013. Pengaruh Lingkungan terhadap Komposisi Kimia dan Aktivitas Antioxidant pada Spons Laut. Jurnal Ekologi Lingkungan. 32(4), 121-128.

Zuhra, C. F., Juliati Br. Tarigan, Herlince Sihotang. 2008. Aktivitas Antioksidan Senyawa Flavonoid dari Daun Katuk (Sauropus androgunus (L) Merr.). Jurnal Biologi Sumatera, Departemen Kimia FMIPA, USU 3 (1): 7-10.