Antibacterial Activity Test Of Kecombrang Fruit (Etlingera elatior) Extracted Using Multiple Solvents

Abstract

The purpose of this study was to test the antibacterial activity of kecombrang fruit (Etlingera elatior) extracted using H₂O, ethyl acetate, and n-hexane as solvents. This study used a descriptive method with the type of solvent and kecombrang powder treatment respectively 25g:250g. The observation procedure was the calculation of the yield and diameter of the inhibition zone against Escherichia coli bacteria. Based on the yield calculation, it is known that H₂O, ethyl acetate, and n-hexane have average yield values of 11.8166%, 7.5536%, and 1.3038%, respectively. Antibacterial activity using ethyl acetate solvent resulted in an inhibition zone diameter of 33 mm, then H₂O solvent produced 28.25 mm while n-hexane did not have an inhibition zone diameter.

Keywords:  Kecombrang, Extraction, Yield, Antibacterial

Abstrak

Tujuan dari penelitian ini yaitu untuk menguji aktivitas antibakteri buah kecombrang (Etlingera elatior) yang diekstrak dengan menggunakan pelarut H₂O, etil asetat, dan n-heksana. Penelitian ini menggunakan metode deskriptif dengan perlakuan jenis pelarut dan serbuk kecombrang masing-masing 25g:250g. Prosedur pengamatan yaitu perhitungan rendemen dan diamater zona hambat terhadap bakteri Escherichia coli. Berdasarkan hasil perhitungan rendemen diketahui bahwa H₂O, etil asetat, dan n-heksana memiliki nilai rata-rata rendemen berturut-turut 11,8166%, 7,5536%, dan 1,3038%. Aktivitas antibakteri menggunakan pelarut etil asetat menghasilkan diameter zona hambat 33 mm, kemudian pelarut H₂O menghasilkan 28,25 mm sedangkan n-heksana tidak memiliki diameter zona hambat.

Kata kunci: Kecombrang, Ekstraksi, Rendemen, Antibakteri