Analisis Degradasi Awal Plastik PET oleh Bakteri Stenotrophomonas sp. dari Teluk Manado
DOI:
https://doi.org/10.35800/jip.v14i2.69527Keywords:
biodegradation, FT-IR, PET plastic, SEM, Stenotrophomonas sp.Abstract
Indigenous bacteria have the potential to degrade plastic, as demonstrated by the successful isolation of Stenotrophomonas sp. from the waters of Manado Bay, North Sulawesi. This study aimed to evaluate the ability of Stenotrophomonas sp. to degrade polyethylene terephthalate (PET) and to investigate the initial indications of PET degradation. A stock culture of Stenotrophomonas sp. maintained in the laboratory was revitalized in Nutrient Broth (NB) medium and recharacterized morphologically on agar medium. The degradation assay used PET specimens measuring 1 × 1 cm, incubated in NB medium as the control and NB inoculated with Stenotrophomonas sp. as the treatment. The cultures were incubated in a shaker incubator at 37°C for 120 days. Degradation was evaluated based on the percentage of PET weight loss. Changes in surface morphology and chemical structure were analyzed using Scanning Electron Microscopy (SEM) and Fourier Transform Infrared (FT-IR) spectroscopy, respectively. The results showed a greater weight reduction in PET inoculated with Stenotrophomonas sp. than in the control. SEM analysis revealed aggregates, potentially corresponding to bacterial colonies or residual biofilm, as well as cracks indicating structural damage. FT-IR analysis showed a slight decrease and broadening of the carbonyl band and a reduction in the asymmetric and symmetric stretching bands of the ester C–O–C groups. These findings suggest that Stenotrophomonas sp. may contribute to the biodegradation of PET plastic.
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