KARAKTERISASI DAN AKTIVITAS ANTIOKSIDAN SERAT PANGAN DARI DAGING BUAH PALA (MYRISTICA FRAGRANS Houtt)
Abstract
Tujuan dari penelitian ini untuk mengetahui karakteristik fisikokimia dan potensi antioksidan serat pangan dari daging buah pala. Parameter yang digunakan adalah komposisi proksimat, serat pangan, kandungan hemiselulosa, selulosa, lignin, karakteristik gugus fungsi, aktivitas antioksidan, dan kapasitas penangkal nitrit. Hasil karakteristik secara fisik dengan analisis Fourier Transform Infra Red (FT-IR) menunjukkan adanya daerah serapan gugus –OH, -CH, -CH2, C-O-C yang merupakan identifikasi adanya selulosa pada sampel. Hasil analisis Particle Size Analysis (PSA) menunjukkan ukuran partikel dari daging buah pala 137.08 µm. Analisis X-ray Difraction (XRD) menunjukkan adanya karakteristik dari selulosa dari sampel daging buah pala. Hasil karakterisasi secara kimia menunjukkan komposisi kimia dari daging buah pala seperti air (9.11%), abu (3.43%), lemak (1.81%), protein (4.04%), serat kasar (17.57%), serat pangan tak larut (48.61%), serat pangan terlarut (1.67%), serat pangan total (50.28%), hemiselulosa (10.72%), selulosa (15.66%) dan lignin (19.09%). Hasil pengujian aktivitas antioksidan menunjukkan bahwa ekstrak fenolik bebas daging buah pala lebih besar dibandingkan dengan ekstrak fenolik terikat daging buah pala dan aktivitas penangkal nitrit dari daging buah pala menunjukkan bahwa ekstrak fenolik bebas daging buah pala lebih besar dibandingkan dengan ekstrak fenolik terikat daging buah pala.
ABSTRACT
The purpose of this study was to determine the physicochemical characteristics and antioxidant potential of food fiber from nutmeg meat. The parameters used are proximate composition, dietary fiber, hemicellulose content, cellulose, lignin, functional group characteristics, antioxidant activity, and nitrite repellent capacity. The results of physical characteristics with Fourier Transform Infra Red (FT-IR) analysis showed the absorption area of the -OH, -CH, -CH2, C-O-C groups which was an identification of cellulose in the sample. The results of the Particle Size Analysis (PSA) show the particle size of the nutmeg flesh 137.08 µm. X-ray Difraction (XRD) analysis showed the characteristics of cellulose from nutmeg meat samples. Chemical characterization results showed the chemical composition of nutmeg meat such as water (9.11%), ash (3.43%), fat (1.81%), protein (4.04%), crude fiber (17.57%), insoluble food fiber (48.61% ), dissolved food fiber (1.67%), total food fiber (50.28%), hemicellulose (10.72%), cellulose (15.66%) and lignin (19.09%). The results of antioxidant activity testing showed that the extract of phenolic-free nutmeg was greater than the phenolic extract bound to nutmeg meat and the nitrite-antidote activity of nutmeg showed that the phenolic extract of nutmeg-free flesh was greater than that of phenolic extract bound to nutmeg meat.
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PDFDOI: https://doi.org/10.35799/cp.13.1.2020.29661
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