Optimizing Thin Layer Chromatography (TLC) Eluent Composition for Compound Content Separation the Ethanolic Extract of Sponge and Ascidia
DOI:
https://doi.org/10.35800/jip.v12i2.57116Keywords:
Eluent, Marine Invertebrate, Optimation, Thin Layer Chromatography (TLC)Abstract
An optimization study of the KLT eluent composition was carried out on the ethanolic extracts of 3 ascidian samples (Ascd-2, Ascd-3, Ascd-4) and 2 sponge samples (Spons-2 and Spons-3). The Ascidia and Sponge marine invertebrate samples were collected from Malalayang, Manado City's coastal waters, and were not identified. This research is a preliminary test to determine the best eluent composition to separate the compounds in the ascidian and sponge sample extracts. Extracted samples with ethanol solvent using the maceration method for 3 x 24 hours at room temperature followed by evaporation at 400C to obtain a thick ethanol extract. The composition of the eluent used is as follows: Hexane: Ethyl Acetate = 3: 7, Hexane: Ethyl Acetate = 2: 3, Hexane: Ethyl Acetate = 1: 9, Methanol: Ethyl Acetate = 1:1, and 100% Methanol. KLT analysis of the extracts from the ascidia and sponge samples showed that most of the variations in eluent composition used could not separate the compound components in the extracts, except the 100% methanol composition which could separate the compounds in the spongs 3 samples.
Keywords : Eluent ; Marine Invertebrate; Optimation ; TLC
Abstrak
Telah dilakukan studi optimasi komposisi eluen KLT terhadap ekstrak etanol dari 3 sampel Ascidia (Ascd-2,Ascd-3,Ascd-4) dan 2 sampel Spons (Spons-2 dan Spons-3). Sampel avertebrata laut Ascidia dan Spons berasal dari perairan pantai Malalayang, Kota Manado dan belum diidentifikasi jenisnya. Penelitian ini merupakan suatu uji pendahuluan yang bertujuan menentukan komposisi eluen terbaik untuk memisahkan senyawa yang terkandung dalam ekstrak sampel ascidian dan spons. Ekstraksi sampel dengan pelarut etanol menggunakan metode maserasi selama 3 x 24 jam pada suhu ruang dan kemudian dievaporasi pada suhu 400C untuk mendapatkan ekstrak etanol kental. Adapun komposisi eluen yang digunakan adalah sebagai berikut: Heksana : Etil Asetat= 3 : 7, Heksana : Etil Asetat = 2 : 3, Heksana : Etil Asetat = 1 : 9, Metanol : Etil Asetat = 1 : 1, dan Metanol 100%. Analisis KLT terhadap ekstrak sampel ascidia dan spons memperlihatkan bahwa sebagian besar variasi komposisi eluen yang digunakan tenyata tidak dapat memisahkan komponen senyawa dalam ekstrak, kecuali komposisi 100% methanol yang dapat memisahkan senyawa pada sampel Spons-3.
Kata Kunci : Avertebrata Laut; Eluen; KLT: Optimasi
References
Blunt, J.W., Copp, B.R., Keyzers. R.A., Munro, M.H.G. & Prinsep, M.R. (2017). Marine Natural Products, Nat.Pro Rep, Issue 3. (34) : 235-294.
Carroll, A.R., Copp, B.R., Davis, R.A., Keyzers, R.A. & Prinsep, M.R. (2019). Marine Natural Products. Nat. Prod. Rep. (36) : 122-173
Harborne, J.B. (1996). Metode Fitokimia: Penentuan Cara Modern Menganalisa Tumbuhan.(Edisi Kedua). Terjemahan oleh Kosasih Padmawinata dan Iwang Soediro. Bandung: ITB.
Kowalska, T. & Sajewicz, M. (2022) Thin-Layer Chromatography (TLC) in the Screening of Botanicals–Its Versatile Potential and Selected Applications. Molecules 27, 6607. https://doi.org/10.3390/ molecules27196607
Luissandy, Sumilat D,A. & Lintang, R.A.J. (2017). Bioaktivitas Antibakteri Fraksi ODS Spons Agelas sp. dari Perairan Pangalisang Pulau Bunaken. Jurnal Pesisir dan Laut Tropis. 2(1): 22-30.
Losung, G., Losung F., Lintang, R.A.J., Wullur, S. & Manoppo, H. (2022). Aktivitas Antibakteri Dari Spons Asal Perairan Pulau Bantong Bolaang Mongondow Timur. Jurnal Pesisir dan Laut Tropis. 10 (1), 81-88
Malintoi, A., Rumengan, I.F.M., Roeroe, K.A., Warouw, V., Rondonuwu, A.B. & Ompi, M. (2020). Komunitas Ascidia di Pesisir Malalayang Dua, Teluk Manado, Sulawesi Utara. Jurnal Pesisir dan Laut Tropis, 8(1): 39-46
Maradou, R.B., Losung, F., Mangindaan, R.E.P., Lintang, R.A.J., Pelle, W.E. & Sambali, H. (2019). Uji Aktivitas Antibakteri Beberapa Spons dari Perairan Salibabu Kepulauan Talaud. Jurnal Pesisir dan Laut Tropis. 7(3):234-241
Opa, L.S., Bara, R.A., Gerung, G.S., Rompas, R.M., Lintang, R.A.J. & Sumilat, D.A. (2018). Uji Aktivitas Antibakteri Fraksi N-Heksana, Metanol dan Air dari Ascidia Lissoclinum sp. Jurnal Pesisir dan Laut Tropis. 1(1): 69-80
Obradović, D., Oljačić, S., Nikolić, K. & Agbaba, D. (2017) Influence of selected mobile phase properties on the TLC retention behavior of ziprasidone and its impurities, Journal of Liquid Chromatography & Related Technologies, 40:5-6, 247-251, DOI: 10.1080/10826076.2017.1298026
Obradović, D. Filipić, S. Nikolić, K. Čarapić, M. & Agbaba, D. (2016). Optimization of TLC Method for Separation and Determination of Ziprasidone and its Impurities. J. Liq. Chromatogr. Relat. Technol. 39, 271–276
Pasodung, A., Losung, F., Angkouw, E.D., Lintang, R.A.J., Mantiri, D.H.M., Sumilat, D.A. (2018). Uji Aktivitas Antibakteri Spons Plakortis sp. yang Dikoleksi dari Perairan Bunaken. Jurnal Pesisir dan Laut Tropis. 1(1):44-51.
Palit, C., Sumilat, D. A., Rumengan, A. P., Boneka, F. B., Sinjal, C. A. and Lalita, J. (2022). Komunitas dan Keanekaragaman Ascidia di Pesisir Minanga, Malalayang Satu, Kota Manado, JURNAL PESISIR DAN LAUT TROPIS, 10(2), pp. 115–121.
Rachmawati, F. (2015). Optimasi Penggunaan Kromatografi Lapis Tipis (KLT) Pada Pemisahan Senyawa Alkaloid Daun Pulai (Alstonia scholaris L.R.Br). Skripsi. UIN Maulana Malik Ibrahim. Malang.
Rompas, R. (2011). Farmakognosi Laut (Sumber Baru Ekonomi Kelautan). Dewan Kelautan Indonesia, Jakarta.
Saputri, N. D. (201)6. Analisis Kadar Flavonoid pada Benalu kopi (Dendrophthoe pentandra) menggunakan Teknik Kromatografi Lapis Tipis-Densitometri. Skripsi. FMIPA-Universitas Jember
Sumilat, D.A., Lintang, R.A.J., Undap, S.L., Adam, A.A., & Tallei, T.E. (2022) Phytochemical, antioxidant, and antimicrobial analysis of Trichoderma asperellum Eudistoma isolated from ascidian sp. J Appl Pharm Sci, 12(04):090–095.
Downloads
Published
How to Cite
License
Copyright (c) 2024 Rosita Lintang, Fitje Losung, Febry I. S. Menajang, Deiske Adeliene Sumilat
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
COPYRIGHT
Authors who publish with this journal agree to the following terms:
Authors hold their copyright and grant this journal the privilege of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that permits others to impart the work with an acknowledgment of the work's origin and initial publication by this journal.
Authors can enter into separate or additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (for example, post it to an institutional repository or publish it in a book), with an acknowledgment of its underlying publication in this journal.
Authors are permitted and encouraged to post their work online (for example, in institutional repositories or on their website) as it can lead to productive exchanges, as well as earlier and greater citation of the published work (See The Effect of Open Access).
