DNA Barcoding Dalugha (Cyrtosperma Merkusii) di Kepulauan Talaud dan Minahasa Selatan Berdasarkan Gen rbcL
DOI:
https://doi.org/10.35799/jbl.v11i2.34452Keywords:
Dalugha, DNA barcoding, gen rbcLAbstract
(Article History: Received June 28, 2021; Revised August 30, 2021; Accepted Sept 3, 2021)
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ABSTRAK
Dalugha (Cyrtospera merkusii (Hassk.)Schott) merupakan tanaman endemik Sulawesi Utara yang digunakan sebagai pangan alternatif (penganti beras). Penelitian ini bertujuan untuk membandingkan spesies daluga di Kepulauan Talaud dan Minahasa Selatan menggunakan DNA barcode gen rbcL (ribulose 1,5 bisphosphate carboxylase large). Perbandingan barcode DNA yang dilakukan pada empat sampel yang berbeda lokasi tersebut keduanya menghasilkan tingkat kesamaan 100% (identik). Dengan demikian, tidak ada variasi intra spesies yang ditemukan dari semua sampel yang ada. Selanjutnya, kemiripan sampel-sampel ini telusuri kemiripannya dengan kerabat terdekat yang tercatat di GenBank menggunakan BLAST (Basic Local Alignment Search Tool). Tanaman dalugha dalam penelitian ini memiliki kemiripan 99,82% dengan tumbuhan Anaphyllopsis americana (AM905753.1), dan kemiripannya 99,63% dengan Cyrtosperma macrotum (AM905750.1), Lasimorpha senegalensis (AM905755.1), Pycnospatha arietina (AM905751.1), dan Podolasia stipitata (AM905752.1). Belum ada rekor sekuens DNA gen rbcL dari spesies ini yang dibisa dibandingkan di GenBank.
Kata Kunci: Dalugha; DNA barcoding; gen rbcL
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ABSTRACT
Dalugha (Cyrtospera merkusii (Hassk.) Schott) is an endemic plant in North Sulawesi that is used as alternative food (substitute for rice). This research aimed to compare the DNA barcode of dalugha in Talaud Islands and in South Minahasa using rbcL (ribulose 1,5 bisphosphate carboxylase large) gene. The DNA barcoding comparison of all four samples in both area resulted in 100% similarity (identical). Therefore, there is no intraspecific variation found in all samples. Furthermore, the similarity of these samples were conducted with BLAST (Basic Local Alignment Search Tool) to compare with its closest relatives in GenBank. The closest relatives of this plant, based on similarity information, are 99.82% with Anaphyllopsis americana (AM905753.1) and all 99.63% with Cyrtosperma macrotum (AM905750.1), Lasimorpha senegalensis (AM905755.1), Pycnospatha arietina (AM905751.1), and Podolasia stipitata (AM905752.1). There is no record yet of rbcL gene sequence of C. merkusii in GenBank for comparison.
Keywords: Dalugha; DNA barcoding; rbcL gene
References
Agustina DN, Faridah, Jenie BSL, (2016) Pengaruh Retrogradassi dan perlakuan kelembaban panas terhadap kadar pati resisten tipe 3. Daluga. Jurnal Teknologi dan Industri Pangan. 27:78-86.DOI:10,6066/Jtip.2016.27.1.78.
Altschul, SF., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool." J. Mol. Biol. 215: 403-410.
Ardiana D, 2009. Teknik Isolasi DNA genom tanaman papaya dan jeruk denganmenggunakan modifikasi Buffer Ctab.Buletin teknik Pertanian 14(1):12-16.
Consortium Barcode of Life (CBOL). (2009). A DNA Barcode for Land Plants. PNAS, 106 (31).
Edgar, RC (2004) MUSCLE: Multiple sequence aligment with high accuracy and high throughout. Nucleic Acid Research 5:1792-1797.
Fazekas, AJ, Burgess KS, Kesanakurti,PR,Graham SW, Newmaster SG, Husband BC,Percy DM,Hajibabaei M, Barret S C H, 2008. Multiple multicolus DNA barcodes from the plastid genome discrimate plant species equally well. Plos one 3: 2802.
Hollingsworth PM, Forrest LL, Spouge JL, Hajibabaei M, Ratnasingham R. 2009. A DNA barcode for land plants. Proc. Natl. Acad. Sci. USA. 106: 12794 – 12797.
Julianti E., Pinaria, A., Lengkong EF, Kolondam BJ. 2015. DNA Barcoding Tanaman Daluga (Cyrtosperma spp) dari Kepulauan Sangihe berdasarkan Gen matK. Jurnal Bio Logos 5(2): 47-52.
Kearse, M., R. Moir, A. Wilson, S. Stones-Havas, M. Cheung, S. Sturrock, S. Buxton, A. Cooper, S. Markowitz, C. Duran, T. Thierer, B. Ashton, P.
Meintjes, and A. Drummond (2012) Geneious Basic: An Integrated and Extendable Desktop Software Platform for the Organization and Analysis of Sequence Data. BIOINFORMATICS 28(12), 1647-1649.
Kress WJ, Erickson DL, 2007. A two-locus global DNA barcode for land plants, the coding rbcl gene complements with the no coding trnH-psbA spacer region.Plos One 2(6) Doi:10,1371/0000508.
Newsmaster SG,Ragupathy S (2009). Testing plant barcoding in a sister complex of Pantropical acacia (Mimosaidea,Fabaceae). Molecular Ecology Resources 9:172-182.
Rao, S; M. Taylor, A.Jokhan, 2014. A descriptor list for giant swamp taro (Cyrtosperma merkusii) and its cultivars in the Federated States of Micronesia. TELOPEA. 16:95-117.
Rahayu, DW; Jannah, M. 2019. DNA Barcode Hewan dan Tumbuhan Indonesia.Yayasan Inspirasi Ide Berdaya.
Tallei, T., Kolondam BJ. 2015. DNA Barcoding of Sangihe Nutmeg (Myristica fragrans) using matK Gene. HAYATI Journal of Biosciences 22(1): 41-47.
Taulu, L.P., Layuk, 2014. Dukungan teknologi untuk mempekuat daya saing pangan lokal di Sulawesi Utara. Buku Memperkuat daya saing produk pertanian. IAARD Press. 389-400.
Virgilio M, Jordaens K, Breman F. 2012. Turning DNA barcodes into an alternative tool for identification African fruits, files as a model (poster). Consorsium Barcode of Life (CBOL).
