Effect of NAA and BAP on Callus Induction of Binahong Internodes (Anredera cordifolia (Ten.) Steenis) under Different Light Conditions
DOI:
https://doi.org/10.35799/jbl.v16i1.68135Keywords:
binahong, callus induction, NAA, BAP, lightingAbstract
Binahong is a medicinal plant containing secondary metabolites with antibacterial and anti-inflammatory activities. The callus culture method is one of the commonly used methods to increase the content of secondary metabolites. The effect of plant growth regulators and light conditions on binahong internodes callus induction remains limited. The aimed of this study was to determine the optimal combination of concentrations of the growth regulators NAA and BAP to induce callus from binahong internode explants in vitro under different lighting conditions. This study employed a randomized block design (RBD) with two factors: light (light and dark) and NAA (0; 1; 2; 3) ppm and BAP (0; 0.5; 1; 1.5) ppm. The parameters observed included callus emergence time, percentage of callus-forming explants, fresh weight, dry weight, and callus morphology. The results showed a significant interaction between the plant growth regulators and lighting conditions for all parameters. The fastest callus formation time (6,8 DAP) occurred with BAP 0.5 ppm + NAA 2–3 ppm under light conditions, the highest percentage under dark conditions (95,25%) while under light conditions (92.94%), and the highest fresh and dry weights were observed with BAP 1.5 ppm + NAA 3 ppm (light) at 0.4128 g fresh weight and 0.0219 g dry weight. The callus was predominantly compact in texture, ranging in color from greenish-white to brown under light conditions and from white to brown under dark conditions. These findings indicate the role of NAA, BAP, and light in binahong internodes callus induction.
References
Abou El-Dis, G. R., Zavdetovna, K. L., Nikolaevich, A. A., Abdelazeez, W. M. A., & Arnoldovna, T. O. (2021). Influence of light on the accumulation of anthocyanins in callus culture of Vaccinium corymbosum L. cv. Sunt Blue Giant. Journal of Photochemistry and Photobiology, 8, 100058. https://doi.org/10.1016/J.JPAP.2021.100058
Adhikari, S. R., & Pant, B. (2013). Induction and proliferation of in vitro mass of callus of Withania somnifera (L.) Dunal. Research in Plant Sciences, 1(3), 58–61. https://doi.org/10.12691/plant-1-3-2
Adiyasa, M., & Meiyanti, M. (2021). Utilization of traditional medicine in Indonesia: distribution and influential demographic factors. Jurnal Biomedika Dan Kesehatan, 4(3), 130–138. https://doi.org/10.18051/JBiomedKes.2021
Al qamari, M., Tarigan, D. M., & Alridiwirsah. (2020). Budidaya Tanaman Obat dan Rempah.
Ali, M., & Abbasi, B. H. (2014). Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L. Journal of Photochemistry and Photobiology B: Biology, 140, 223–227. https://doi.org/10.1016/J.JPHOTOBIOL.2014.08.008
Anwar, N., & Isda, M. (2020). Response Formation of Gotu Kola Leaf Callus (Centella Asiatica (L.) Urb.) with Addition of Naphtalene Acetic Acid and Benzyl Amino Purin In Vitro. Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 5(2), 136–142. https://doi.org/10.24002/biota.v5i3.3232
Ariany, S. P., Sahiri, N., & Syakur, A. (2013). Pengaruh Kuantitas Cahaya terhadap Pertumbuhan dan Kadar Antosianin Daun Dewa (Gynura Pseudochina (L.) Dc) secara In Vitro. Agrotekbis, 1(5).
Cahayani, S., & Habibah, N. (2025). Pertumbuhan Kalus Batang Binahong (Anredera cordifolia) Pada Medium Ms Dengan Penambahan Pikloram Dan Kinetin Dalam Kondisi Gelap Terang. [Skripsi]. Universitas Negeri Semarang.
Chen, Y. M., Huang, J. Z., Hou, T. W., & Pan, I. C. (2019). Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia. Botanical Studies, 60(1). https://doi.org/10.1186/S40529-019-0257-Y
Damanik, I. T. S., Rosmayati, & Siregar, L. A. M. (2017). The Effect of Explants Type and Growth Regulators Composition on The Callus Induction of Binahong (Anredera cordifolia (Ten.) Steenis). JURNAL AGROTEKNOLOGI, 5(3), 532–536. https://doi.org/10.32734/JA.V5I3.2218
Fitriyah, N., Purwa, M. K., Alfiyanto, M. A., Mulyadi, Wahuningsih, N., & Kismanto, J. (2013). Obat herbal antibakteri ala tanaman binahong. Jurnal Kesehatan Kusuma Husada, 4(2), 116–122.
Ghasemi, A., & Zahediasl, S. (2012). Normality Tests for Statistical Analysis: A Guide for Non-Statisticians. International Journal of Endocrinology and Metabolism, 10(2), 486. https://doi.org/10.5812/IJEM.3505
Hidayat, R., Abdullah, A., & Aminah, A. (2021). Pemantapan Masa Inkubasi Kalus Kedelai Secara In Vitro Dalam Media Ms (Murashige dan Skoog) B5 (Gamborg). AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian, 2(2), 60–70. https://doi.org/10.33096/AGROTEKMAS.V2I2.192
Jannah, I., & Nurhidayah, T. (2024). The Effect of Leaf Position and Combinations of BAP and NAA on the Development of Sansevieria downsii Leaf Tissues Explant. JURNAL AGROTROPIKA, 23(2), 257–269. https://doi.org/10.23960/JA.V23I2.8473
Joshi, K., Chaturvedi, P., & Shubhpriya, S. (2013). Efficient in vitro regeneration protocol of Centella asiatica (L.) Urban: an endemic and underutilized nutraceutical herb. African Journal of Biotechnology, 12(33), 5164–5172. https://doi.org/10.5897/AJB2013.12817
Junairiah, J., Purnomo, P., & Utami, E. (2018). Callus Induction of Piper betle L. Var Nigra Using 2, 4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin. Biosaintifika: Journal of Biology & Biology Education, 10(3), 588–596. https://doi.org/10.15294/biosaintifika.v10i3.15962
Kadapi, M., Sunarso, C., Erizon, M. S., Maharani, N. D., Hakim, M. S., & Zahra, I. H. A. (2024). Teknologi Kultur in vitro untuk Meningkatkan Produksi Metabolit Sekunder pada Berbagai Tanaman Obat. Jurnal AGROSAINS Dan TEKNOLOGI, 9(1), 18–29. https://doi.org/10.24853/JAT.9.1.18-29
Khoiriyah, S., Santosa, D., & Purwantini, I. (2023). Effects of Combination of 2,4 D and Kinetin on Callus Formation of Catharanthus roseus (L.) G. Don Leaves and Detection of Alkaloid Content. Majalah Farmaseutik, 19(3), 385–393. https://doi.org/https://doi.org/10.22146/farmaseutik.v19i3.82593
Kristianto, A., & Setyorini, T. (2021). Induksi kalus eksplan daun lada (Piper nigrum L.) pada modifikasi media MS dengan penambahan hormon NAA dan BAP. Agritech: Jurnal Fakultas Pertanian Universitas Muhammadiyah Purwokerto, 23(2), 160–166. https://doi.org/https://doi.org/10.30595/agritech.v23i2.12028
Kumalasari, E., & Sulistyani, N. (2011). Antifungal Activity Of Ethanol Extractof Binahong Stem (Anredera cordifolia (Tenore)Steen) Against Candida albicans And Thephytochemical Screening. Jurnal Ilmiah Kefarmasian, 1(2), 51–62. https://doi.org/https://doi.org/10.12928/pharmaciana.v1i2.524
Latifah, R., Suhermiatin, T., & Ermawati, N. (2017). Optimasi pertumbuhan plantlet Cattleya melalui kombinasi kekuatan media Murashige-Skoog dan bahan organik. Journal of Applied Agricultural Sciences, 1(1), 59–62. https://doi.org/10.25047/agriprima.v1i1.20
Mahadi, I., Syafi’i, W., & Sari, Y. (2016). Callus Induction of Calamansi (Citrus microcarpa) Using 2,4-D and BAP Hormones by in vitro Methods. Jurnal Ilmu Pertanian Indonesia, 21(2), 84–89. https://doi.org/10.18343/jipi.21.2.84
Mulyono, M., Salsabila, M. S., Rasnijal, M., Fadilah, S., & Putra, A. (2025). Callus Induction in Kappaphycus alvarezii Using Indole-3-Acetic Acid (IAA) and 6-Benzylaminopurine (BAP) for Seedstock Development. Indonesian Aquaculture Journal, 20(1), 1–10. https://doi.org/http://dx.doi.org/10.15578/iaj.20.1.2025.1-10
Paryatin, P., Setiti Wida Utami, E., Sri Wulan Manuhara, Y., & Pujiastuti, P. (2024). The effect of light and growth regulators on callus induction and growth in Erythrina lithosperma Miq. International Journal of Frontiers in Biology and Pharmacy Research, 06(02), 021–027. https://doi.org/10.53294/ijfbpr.2024.6.2.0042
Prasetyo, Y., Njudang, K., Wibowo, H. M. P., & Krisnawan, A. H. (2020). Evaluasi Pertumbuhan Suspensi Sel Dendrobium anosmum var. gigantea dan Aktivitasnya sebagai Antioksidan. Media Pharmaceutica Indonesiana, 3(2), 70–79. https://doi.org/10.24123/mpi.v3i2.2976
Purnamaningsih, R., & Ashrina, M. (2011). the Effect of Bap and Naa on Callus Induction and Artemisinin Content in Artemisia annua L. Berita Biologi, 10(4), 481–489.
Puteri, R. F. (2014). The Effect of Different Concentration of NAA (Napthalene Acetic Acid) and BAP (Benzyl Amino Purine) on Soursop Leaves Callus Induction (Annona muricata L) In Vitro. LenteraBio: Berkala Ilmiah Biologi, 3(3), 154–159.
Rahayu, B., Solichatun, & Anggarwulan, E. (2003). The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on callus growth and production flavonoid content on culture callus Acalypha indica L. Biofarmasi, 1(1), 1–6.
Safitri, S. K., Siregar, L. A., & Lubis, K. (2017). Induction Callus of Roselle (Hibiscus sabdariffa Linn.) on The Explants Type and Different Concentration of Auxin. Jurnal Agroekoteknologi FP USU, 5(3), 593–598. https://doi.org/https://doi.org/10.32734/ja.v5i3.2222
Salsabila, M. J., & Isda, M. N. (2022). Callus Induction from Leaf Explants Of Tacca (Tacca chantrieri Andre) On Murashige and Skoog Media with Different Sucrose Concentrations In Vitro. Jurnal Biologi UNAND, 10(1).
Sari, Y., Kusumawati, E., Saleh, C., & Sukartingsih, S. (2018). Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa. Nusantara Bioscience, 10(3), 183–193. https://doi.org/https://doi.org/10.13057/nusbiosci/n100309
Setiawati, T., Arofah, A. N., & Nurzaman, M. (2020). Induksi Kalus Krisan (Chrysanthemum morifolium Ramat var. Tomohon Kuning) Dengan 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) pada Kondisi Pencahayaan Berbeda. Pro-Life, 7(1), 13–26. https://doi.org/10.33541/pro-life.v7i1.1541
Setiawati, T., Ayalla, A., & Witri, A. (2019). Induksi kalus krisan (Chrysanthemum morifolium Ramat.) dengan penambahan berbagai kombinasi zat pengatur tumbuh (ZPT). EduMatSains: Jurnal Pendidikan, Matematika Dan Sains, 3(2), 119–132.
Suhartanto, B., Astutik, M., Umami, N., Suseno, N., & Haq, M. S. (2022). The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.). Iopscience.Iop.OrgB SuhIOP Conference Series: Earth and Environmental Science, 1001(1). https://doi.org/10.1088/1755-1315/1001/1/012006/META
Sulichantini, E. (2015). Produksi metabolit sekunder melalui kultur jaringan. Proceeding of Mulawarman Pharmaceuticals Conferences, 1, 205–212. https://doi.org/https://doi.org/10.30872/mpc.v1i.158
Swandari, T., & Setyorini, T. (2018). In vitro callus induction of Gerbera jamesonii with combination of NAA And BAP. AGROISTA: Jurnal Agroteknologi, 1(2).
Syabana, M., Marianingsih, P., Hermita, N., & Rohimah, I. (2017). In Vitro Callus Induction and Growth of Stevia (Stevia rebaudiana Bertoni M.) with Difference Concentrations of PEG (Polyethylene Glycol) and Light Conditions. Biodidaktika: Jurnal Biologi Dan Pembelajarannya, 12(2), 57–68. https://doi.org/http://dx.doi.org/10.30870/biodidaktika.v12i2.2330
Vishal Gupta, N., & Shukshith, K. S. (2016). Qualification of Autoclave. International Journal of PharmTech√ Research, 9(4), 220–226.
Wahyuni, D. K., Huda, A., Faizah, S., Purnobasuki, H., & Wardojo, B. P. E. (2020). Effects of light, sucrose concentration and repetitive subculture on callus growth and medically important production in Justicia gendarussa Burm.f. Biotechnology Reports, 27, e00473. https://doi.org/10.1016/J.BTRE.2020.E00473
Walangadi, F. R., Ahmad, J., Pagalla, D. B., Kandowangko, N. Y., & Febriyanti, F. (2025). Effect of BAP and NAA on Callus Emergence Time of Dumbaya Young Leaf Explants in Vitro. Jurnal Biologi Tropis, 25(2), 1903–1911. https://doi.org/10.29303/jbt.v25i2.9045
Wonganu, B. (2007). Callus Induction of Beet Root for Speed up Economical Plant Production. The Journal of KMITNB. Thailand, 17(2), 21–26.
Wulandari, D., Utami, E., & Zuraidassanaaz, N. (2021). Callus induction and secondary metabolite profile from Elephantopus scaber L. Journal of Tropical Biodiversity and Biotechnology, 6(1). https://doi.org/https://doi.org/10.22146/JTBB.59234
Yanti, D., & Isda, M. (2021). Shoots Induction of nodes (Citrus microcarpa Bunge.) with addition 6- Benzyl Amino Purine (BAP) by In Vitro. Biospecies, 14(1), 53–58. https://doi.org/https://doi.org/10.22437/biospecies.v14i1.11192
Zhang, X., Bernatavichute, Y. V., Cokus, S., Pellegrini, M., & Jacobsen, S. E. (2009). Genome-wide analysis of mono-, di- and trimethylation of histone H3 lysine 4 in Arabidopsis thaliana. Genome Biology, 10(6), R62. https://doi.org/10.1186/GB-2009-10-6-R62
Zhou, J., Wang, X., He, K., Charron, J. B. F., Elling, A. A., & Deng, X. W. (2010). Genome-wide profiling of histone H3 lysine 9 acetylation and dimethylation in Arabidopsis reveals correlation between multiple histone marks and gene expression. Plant Molecular Biology, 72(6), 585–595. https://doi.org/10.1007/S11103-009-9594-7
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Shilfina Ramadani, Noor Aini Habibah
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright Notice
Jurnal Bios Logos is an open access journal and the article's license is CC-BY-NC. This license lets others distribute, remix, tweak, and build upon author's work, as long as they credit the original creation. Authors retain copyright and grant the journal/publisher non exclusive publishing rights with the work simultaneously licensed under a https://creativecommons.org/licenses/by-nc/4.0/ . And Authors can still use their work commercially.
