Analysis Of Types Of Carotenoid Pigments In Crab Sesarmops sp From Manado By Coast
DOI:
https://doi.org/10.35800/jip.9.2.2021.35127Keywords:
Column Chromatography (CC), Carotenoids, Sesarmops spAbstract
Carotenoid pigments are a group of pigments that are yellow, orange, and red-orange in color. Pigments are natural dyes found in plants and animals, extracts of carotenoid pigments can be separated by chromatographic methods where the common chromatographic methods in determining the type of pigment are Column Chromatography (CC) and Thin Layer Chromatography (TLC). The purpose of this study was to determine the types of pigments contained in the carapace extract of the male crab Sesarmops sp. The Sesarmops sp crab has a brown dorsal carapace with blackish-brown leg spots known as mangrove crabs. Crabs in the Sesarmidae family have a carapace formation and wide legs, do not have swimming legs, and have a pair of claws that are faded purple, the presence of these colors can be identified as containing carotenoid pigments. The results of this study obtained the content of carotenoid pigment 25.2 g/gr and the value of the concentration of carotenoid pigment in the male crab extract Sesarmops sp 10.99 g. The results of the separation of the total pigment extract using column chromatography obtained the types of pigments -carotene, Ekinenon, Zeaxanthin, and Astaxanthin.
Â
Keywords: Column Chromatography (CC); Carotenoids;Â Sesarmops sp
Â
                                                                  Abstrak
Pigmen karotenoid adalah sekelompok pigmen yang berwarna kuning, oranye dan merah oranye. Pigmen adalah zat warna alami yang terdapat pada tumbuhan dan hewan, ekstrak pigmen karotenoid dapat dipisahkan dengan metode kromatografi yang dimana metode kromatografi umum dalam menentukan jenis pigmen adalah Kromatografi Kolom (KK) dan Kromatografi Lapis Tipis (KLT). Tujuan dari penelitian ini untuk mengetahui jenis-jenis pigmen apa saja yang terdapar pada ekstrak karapas kepiting jantan Sesarmops sp. Kepiting Sesarmops sp memiliki karapas dorsal bewarna coklat dengan bintik kaki coklat kehitaman yang dikenal sebagai kepiting mangrove. Kepiting dalam keluarga sesarmidae memiliki bentukan karapas dan kaki yang lebar tidak memiliki kaki renang serta memiliki sepasang capit berwarna ungu pudar, adanya warna tersebut kepiting dapat diidentikasikan mengandung jenis pigmen karotenoid.  Dari hasil penelitian ini mendapatkan kandungan pigmen karotenoid 25,2 dan nilai konsentrasi pigmen karotenoid pada ekstrak kepiting jantan Sesarmops sp 10,99 . Hasil pemisahan dari ekstrak pigmen total menggunakan kromatografi kolom didapatkan jenis pigmen β-karoten, Ekinenon, Zeaxantin dan AstaxantinReferences
Abdullah, M.R, Paransa, D.S.J, dan Mantiri, D.M.H. 2018. Distribusi Pigmen Karotenoid Pada Kepiting Grapsus sp. Dengan Menggunakan Metode Kromatografi Lapis Tipis. Jurnal Pesisir dan Laut Tropis. Vol. 2 No. 1 Tahun 2018. Hal 23-24.
Britton, G., Jansen, S.L., and Pfander, H. 1995. Caratenoids. Volume 1B. Spectroscopy. Basel, Switzerland. Hal 347.
Britton, G., Jansen, S.L., and Pfander, H. 2004. Carotenoids handbook. Basel, Switzerland. Birkhauser Veralg AG. 563 hal
Chang, R. 2010. Chemistry. Published The By McGraw-Hill Companies. New York. 1084.
Demarjati dkk,. 1990. Morfologi Invertebrata dan Vertebrata. Jakarta:Tira Pustaka.
Diasasthisa, Paransa, D. S. J., Mantiri D. M. H., Rumengan. A., Warow. V., Silaki. M. (2019). Isolasi Pigmen Karotenoid Dari Kepiting Grapsus sp. Jurnal Pesisir dan Laut Tropis Volume 7, No. 1 2019.
Hadden, W.L., Watkins, R.H., Levy, L.W., Regalado, E., Rivadeneira, D.M,, van Breemen RB., and Schwartz, S.J. 1999. Carotenoid composition of marigold (Tagetes erecta) flower extract used as nutritional supplement. J. Agric. Food. Chem. 47:4189–94.
Mantiri, D. H. M., D.J Paransa dan J.F. Koagow. 2002. Telaah Awal Kandungan Pigmen Karotenoid Pada Kepiting Biola Uca vocans Jantan. Jurnal Perikanan dan Ilmu Kelautan. Vol I. No. I. FPIK. UNSRAT. Hal 43-47
Milne Edwards H. 1837. Histoire naturelle des Crustacés comprenant l’anatomie, la physiologie et la classification de ces animaux. Librairie Encyclopedique de Roret, Paris, Vol. II: 531 pp.; + separate atlas to Vol. II: 32 pp.
Paransa, D.J. D.H.M. Mantiri, F. Korompis. 2002. Penentuan Kandungan Pigmen Karatenoid Pada Kepiting Grapsus albolineatus Betina Berdasarkan Beda Larutan Pengembang Pada Kromatografi Lapis Tipis. Jurnal Perikanan Dan Ilmu Kelautan. Vol 1. No. 3 2002. FPIK. UNSRAT. Hal.1-9.
Paransa, D.S.J., D.M.H. Mantiri., J.J.H.Paulus., C.Lumenta., M.ompi., S. Darwisito., C.Paruntu., A. Kase., A.D.N. Rupidara. 2019. Exploration Study Of The Pigment Types Of Stone Crab (Grapsus albolineatus) as pharmaceutical and Cosmetics Ingridients. Jurnal Eco.Env and Cons (25 August Suppl. Issue). (S1-S6).
Packer, L. 1992. Caratenoids. Part A In Metnods In Enzimology. Volume 213. Academic Press Inc. California.
Poore, C.B Gary. 2004. Marine Decapode Crustacea Of Southern Australia. A Guide To Indentification. CSIRO Publishing. Australia. Hal 574.
Pratiwi R. dan Rahmat. 2015. Sebaran Kepiting Mangrove (Crustacea: Decapoda) yang terdaftar di Koleksi Rujukan Pusat Penelitian Oseanografi-Lipi 1960-1970. Jurnal Ilmu-ilmu Hayati. 14(2): Hal 195-202.
Ruppert E.E and Barnes R. D. 1985. Invertebrate Zoologi. Sixth Edition.
Sagala, L. S. S., Idris, M dan M. N. Ibrahim. 2013. Perbandingan Pertumbuhan Kepiting Bakau ( scylla Seratta) Jantan dan Betina Pada Metode Kurungan Dasar. Jurnal Laut Indonesia. 3 (12).
Salim, K. P., R. Indrawati dan L. Limantara. 2016. Terapi Alternatif penyakit kardiovaskular dengan pigmen alami. CDK-246/Vol. 43 No 11 Tahun 2016. Universitas Ma Chung Malang.
Serène R. (ed) Prodromus for a check list of the non-planctonic marine fauna of South East Asia. UNESCO, Singapore Nat Acad Sci Singapore Spec Publ 1:33–112, pls. 16–17. Serène R, Soh CL. 1970. New Indo-Pacific genera allied to Sesarma Say 1877 (Brachyura, Decapoda, Crustacea). Treubia 27:387– 416.
Sibarani, S. E., Paransa, D. S. J., Kemer,K., Mantiri, D. M., Rumampuk, N. D.,& Tumembow, S. S. 2020. PigmenKarotenoid Pada Kepiting Ozius Sp.Jurnal Pesisir Dan Laut Tropis, 8(1),47-55.
Silaa A. E. 2019. Ekstrak Pigmen Karotenoid Dari Kepiting Grapsus sp. Sebagai Aktivitas Koagulan. Skripsi, Manado. Fakultas Perikanan dan Ilmu Kelautan Program Studi Ilmu Kelautan UNSRAT Manado. Hal.1-32.
Thamin A., C. Umar dan D. Paransa 2006. Analisis Pigmen dan Aktifitas Antibakteri In Vitro Pigmen Astaksanthin Kepiting (Grapsus Albolineatus Lamarck) Jantan. Jurnal Perikanan (J Fish-Sci) VII (2):160-168 ISSN0853-6084.
VÃlchez. C, Forján. E, Cuaresma. M, Bédmar. F, Garbayo. I and Vega. J. M. 2011. Marine Carotenoids: Biological Functions and Commercial Applications. Journal Marine Drugs. Vol 9. Hal : 319-333.
WoRMS (2021). Sesarmops impressionus (H. Milne Edwards, 1837). Diakses di: http://marinespecies.org/aphia.php?p=taxdetails&id=444693
Zeak,W. L., Paransa, D. S. J., Rumengan, A., Kemer K., Paulus J.J.H., Mantiri D.M.H. 2019. Skrining Pigmen Karotenoid Pada Kepiting Grapsus sp. Dengan Menggunakan Pemisahan Kromatografi. Jurnal Pesisir dan Laut Tropis, 1 (1) : 52-58.
Downloads
Published
How to Cite
Issue
Section
License
COPYRIGHT
Authors who publish with this journal agree to the following terms:
Authors hold their copyright and grant this journal the privilege of first publication, with the work simultaneously licensed under a Creative Commons Attribution License that permits others to impart the work with an acknowledgment of the work's origin and initial publication by this journal.
Authors can enter into separate or additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (for example, post it to an institutional repository or publish it in a book), with an acknowledgment of its underlying publication in this journal.
Authors are permitted and encouraged to post their work online (for example, in institutional repositories or on their website) as it can lead to productive exchanges, as well as earlier and greater citation of the published work (See The Effect of Open Access).
