Identifikasi Trichoderma sp. dari Rizosfer Tanaman Kanonang (Chordia dichotoma. G. Forst) dan Karakteristik Pemacu Pertumbuhan serta Daya Antagonismenya Terhadap Fusarium oxysporum

Authors

  • Billy David Rawis Program Studi Magister Biologi, Jurusan Biologi FMIPA UNSRAT
  • Dylan R. Pahlevi Balai Perlindungan dan Pengujian Mutu Tanaman Pangan dan Hortikultura, Kalasey Sulawesi Utara
  • Parluhutan Siahaan Jurusan Biologi FMIPA Universitas Sam Ratulangi Manado
  • Marina Singkoh Jurusan Biologi FMIPA Universitas Sam Ratulangi Manado
  • Agustina Tangapo Jurusan Biologi FMIPA Universitas Sam Ratulangi Manado

DOI:

https://doi.org/10.35799/jbl.v14i3.60630

Keywords:

Trichoderma, Rhizosphere, molecular, Kanonang Plant, Fusarium oxysporum

Abstract

The use of biological control agents to inhibit the growth of pathogens in this case Fusarium in agricultural plants is very important in order to reduce the impact of environmental damage. Trichoderma sp. is a fungus that can be obtained from the rhizosphere of plants that can increase soil fertility and inhibit pathogenic fungi that cause damage to agricultural plants. The use of local isolates of antagonistic fungi in this case from the rhizosphere of the Kanonang plant gave results where the discovery of Trichoderma species that are able to control plant pathogens in this case Fusarium oxysporum through antagonism mechanisms such as inhibition, antibiosis and microparasitism. To find out the species of Trichoderma sp, it was continued with a molecular test using the ITS marker gene and the results of the test confirmed that the Trichoderma species in the rhizosphere of the Kanonang plant was the Trichoderma asperellum type. This type of Trichoderma also after being tested for its characteristics in stimulating growth gave positive results for both phosphate solvent and auxin producer (IAA) tests

References

Adhi, S. R., & Suganda, T. (2020). Potensi jamur rizosfer bawang merah dalam menekan Fusarium oxysporum f.sp. cepae, penyebab penyakit busuk umbi bawang merah. Kultivasi, 19(1), 1015. https://doi.org/10.24198/kultivasi.v19i1.22877

Badan Standarisasi Nasional. (2014). Agens Pengendali Hayati (APH) Beauveria bassiana. Sni 8027.1:2014.

Gusnawaty, Taufik, M., Triana, L., & Asniah. (2014). KARAKTERISASI MORFOLOGIS Trichoderma spp. INDIGENUS SULAWESI TENGGARA Morphological Characterization Trichoderma spp. Indigenous Southeast of Sulawesi. J. Agroteknos, 4(2), 88–94.

Khalid, S. A., & Ramadan, A. B. (2018). Internal transcribed spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita. African Journal of Microbiology Research, 12(30), 715–722. https://doi.org/10.5897/ajmr2018.8915

Khoirunisa, F. R., A. W. Sektiono, S. Djauhari, and L. Q. Aini. “UJI POTENSI JAMUR PENGHASIL IAA SEBAGAI PENGENDALI HAYATI PATOGEN LAYU Fusarium Oxysporum PADA TANAMAN CABAI: INVESTIGATING THE POTENTIAL OF IAA-PRODUCING FUNGUS AS A BIOLOGICAL CONTROL OF FUSARIUM WILT ON CHILI”. Jurnal HPT (Hama Penyakit Tumbuhan), vol. 12, no. 1, Mar. 2024, pp. 25-41, doi:10.21776/ub.jurnalhpt.2024.012.1.3.

Lange, S., Pohl, J., & Santarius, T. (2020). Digitalization and energy consumption. Does ICT reduce energy demand? Ecological Economics, 176(June 2020). https://doi.org/10.1016/j.ecolecon.2020.106760

Manzar, N., Kashyap, A. S., Goutam, R. S., Rajawat, M. V. S., Sharma, P. K., Sharma, S. K., & Singh, H. V. (2022). Trichoderma: Advent of Versatile Biocontrol Agent, Its Secrets and Insights into Mechanism of Biocontrol Potential. Sustainability (Switzerland), 14(19), 1–32. https://doi.org/10.3390/su141912786

Michielse, C. B., & Rep, M. (2009). Pathogen profile update: Fusarium oxysporum. Molecular Plant Pathology, 10(3), 311–324. https://doi.org/10.1111/j.1364-3703.2009.00538.x

Molebila, D. Y., Rosmana, A., & Tresnaputra, U. S. (2020). Trichoderma asal akar kopi dari Alor: Karakterisasi morfologi dan keefektifannya menghambat Colletotrichum Penyebab Penyakit Antraknosa secara in Vitro. Jurnal Fitopatologi Indonesia, 16(2), 61–68. https://doi.org/10.14692/jfi.16.2.61-68

Picardal, J. P., Danielle, E., Tundag, L., Picardal, M. T., & Goc-Ong, G. B. (2019). Antagonistic activity of Metarhizium anisopliae (Metschnikoff) against phytopathogenic Fusarium oxysporum f. sp. cubense (Schlecht.) as a biological control. CNU Journal of Higher Education, 13(November), 25–33.

Prashar, P., Kapoor, N., & Sachdeva, S. (2014). Rhizosphere: Its structure, bacterial diversity and significance. Reviews in Environmental Science and Biotechnology, 13(1), 63–77. https://doi.org/10.1007/s11157-013-9317-z

Sari, W., Wiyono, S., Nurmansyah, A., Munif, A., & Poerwanto, R. (2018). Keanekaragaman dan Patogenisitas Fusarium spp. Asal Beberapa Kultivar Pisang. Jurnal Fitopatologi Indonesia, 13(6), 216. https://doi.org/10.14692/jfi.13.6.216

Susila, E., Maulina, F., & Emilda, D. (2023). Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia. Biodiversitas, 24(4), 2064–2071. https://doi.org/10.13057/biodiv/d240416

Sood, M., Kapoor, D., Kumar, V., Sheteiwy, M. S., Ramakrishnan, M., Landi, M., Araniti, F., & Sharma, A. (2020). Trichoderma: The “secrets” of a multitalented biocontrol agent. Plants, 9(6), 1–25. https://doi.org/10.3390/plants9060762

Syarifah, S. M., Sari, O. P., & Bimantara, A. (2024). Pengendalian Hayati Patogen Fusarium oxysporum f. sp. capsici dengan Isolat Trichoderma sp. Asal Rizosfer Bambu dari Kecamatan Kedu, Kabupaten Temanggung. Jurnal Ilmu Pertanian Indonesia, 29(3), 454–460. https://doi.org/10.18343/jipi.29.3.454

Ulya, H., Darmanti, S., & Ferniah, R. S. (2020). Pertumbuhan Daun Tanaman Cabai (Capsicum annuum L.) yang Diinfeksi Fusarium oxysporum pada Umur Tanaman yang Berbeda. Jurnal Akademika Biologi, 9(1), 1–6.

Wulandari, N., Irfan, M., & Saragih, R. (2020). Isolasi Dan Karakterisasi Plant Growth Promoting Rhizobacteria Dari Rizosfer Kebun Karet Rakyat. Dinamika Pertanian, 35(3), 57–64. https://doi.org/10.25299/dp.2019.vol35(3).4565

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Published

2024-12-30

How to Cite

Rawis, B. D., Pahlevi, D. R., Siahaan, P., Singkoh, M., & Tangapo, A. (2024). Identifikasi Trichoderma sp. dari Rizosfer Tanaman Kanonang (Chordia dichotoma. G. Forst) dan Karakteristik Pemacu Pertumbuhan serta Daya Antagonismenya Terhadap Fusarium oxysporum. JURNAL BIOS LOGOS, 14(3), 141–153. https://doi.org/10.35799/jbl.v14i3.60630

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