The Effect of Addition Various Concentrations of Yeast (Saccharomyces cerevisiae) on the Quality of Porang Tubers (Amorphophallus sp.)

Authors

  • Ira Wati Program Studi Biologi, Jurusan Sains dan Ilmu Formal, Fakultas Sains dan Teknologi, Universitas Bangka Belitung
  • Henny Helmi Program Studi Biologi, Jurusan Sains dan Ilmu Formal, Fakultas Sains dan Teknologi, Universitas Bangka Belitung
  • Rahmad Lingga Program Studi Biologi, Jurusan Sains dan Ilmu Formal, Fakultas Sains dan Teknologi, Universitas Bangka Belitung

DOI:

https://doi.org/10.35799/jbl.v15i3.61693

Keywords:

Calcium Oxalate, Fermentation, Porang Flour, Saccharomyces cerevisiae

Abstract

Porang tubers (Amorphophallus sp.) are a food commodity rich in glucomannan but also contain antinutritional compounds such as calcium oxalate. This study aimed to determine the effect of adding Saccharomyces cerevisiae yeast on improving the quality of porang flour and its ability to reduce calcium oxalate content. The research method used was fermentation with the addition of various concentrations of S. cerevisiae. The treatments applied were soaking with 10% S. cerevisiae, 20% S. cerevisiae, and a control treatment without yeast (0%). Parameters observed during the fermentation process included pH, total acidity, and total population in the porang soaking water. Meanwhile, yield, calcium oxalate, glucomannan, starch, moisture, ash, and color were analyzed to assess porang flour quality. The results showed that pH decreased and total acidity increased with the length of fermentation up to 72 hours. Yeast increased at 10% and 20% S. cerevisiae concentrations until 48 hours and decreased at 72 hours, whereas in the control treatment, population of yeast decreased up to 48 hours and increased at 72 hours. The study indicated that fermentation with S. cerevisiae effectively reduced calcium oxalate content and improved porang flour quality. The lowest calcium oxalate content was found in the 10% S. cerevisiae treatment, while the highest glucomannan content was observed in the 20% treatment. Porang flour treated with 20% S. cerevisiae showed lower moisture and ash content, while flour from the 10% treatment had higher starch content and a brighter color. Based on various test parameters, the porang flour produced in this study complied good quality standards.

References

Aisah, A., Harini, N., & Damat, D. (2021). Pengaruh waktu dan suhu pengeringan menggunakan pengering kabinet dalam pembuatan MOCAF (modified cassava flour) dengan fermentasi ragi tape. Food Technology and Halal Science Journal, 4(2), 172-191.

Alevalia, A., & Arvianti, E. Y. (2023). Analisis Usahatani Porang Sebagai Upaya Diversifikasi Pangan Di Masa Mendatang. Jurnal Ekonomi Pertanian Dan Agribisnis, 7(2), 615-622.

Ambarsari, I., Sarjana, & A. Choliq. (2009). Rekomendasi Dalam Penetapan Standar Mutu Tepung Ubi Jalar. Ungaran.

Arifin, M.A. (2001). Pengeringan Keripik Umbi Ilesiles secara Mekanik untuk Meningkatkan Mutu Keripik Iles. [Tesis]. Bogor : Teknologi Pasca Panen PPS IPB.

Azizah, N., Al-Barrii, A. N., & Mulyani, S. (2012). Pengaruh lama fermentasi terhadap kadar alkohol, pH, dan produksi gas pada proses fermentasi bioetanol dari whey dengan substitusi kulit nanas. Jurnal Aplikasi Teknologi Pangan, 1(3).

Baronil M.D., S. Colombo & E. Martegani. (2018). Antagonism between salicylate and the cAMP signal controls yeast cell survival and growth recovery from quiescence. Dipartimento di Biologia, Università di Padova, Italy. Microbial Cell Vol. 5 No. 7 pp: 344-356.

Bhattacharjee I, Mazumdar D, Saha SP. (2019). Microbial amylases and their potential application in industries: A review. Structure 8: 162- 170.

Buckee, G. K., dan Hargitt, R. (1978). Measurement of carbohydrates in wort and beer, a review. Journal of Institution of Brewing Research. 84(1): 13-21.

BSN. (2020). Serpih Porang. SNI No. 7939:2020. Jakarta: Badan Standarisasi Nasional.

Chapp, A.D.; Shan, Z.; Chen, Q.-H. (2024). Acetic Acid: An Underestimated Metabolite in Ethanol-Induced Changes in Regulating Cardiovascular Function. Antioxidants 2024, 13, 139. https://doi.org/10.3390/ antiox13020139

Chua, M. (2011). An investigation of the biology and chemistry of the chinese medical plant Amorphophallus konjac. Disertasi. University of wolverhampton.

Chavez, A. L. Sanchez, T. Ceballos, H. Rodrigues-Amaya, D.B. Nestel, P. Tohme, J. Ishitani, M. (2006). Retention of Carotenoids in Cassava Roots Submitted to Different Processing Methods. Colombia: John Wiley & Sons, Inc.

Cha´vez-Camarillo GM, Vianey P, Jime RA, Aranda-garcı E, CristianiUrbina E. (2022). Production of extracellular α -amylase by singlestage steady-state continuous cultures of Candida wangnamkhiaoensis in an airlift bioreactor. Plos one 17 (3): e0264734. DOI: 10.1371/journal.pone.0264734.

Dwiyono, K., Sunarti, T. C., Suparno, O., & Haditjaroko, L. (2014). Penanganan Pascapanen Umbi Iles-Iles (Amorphophallus muelleri Blume) Studi Kasus di Madiun Jawa Timur. Jurnal Teknologi Industri Pertanian, 24(3):179-188.

Edam, M. (2017). Aplikasi bakteri asam laktat untuk memodifikasi tepung singkong secara fermentasi. Jurnal Penelitian Teknologi Industri Vol, 9(1), 1-8.

Faridah, A. and Widjanarko, S.B., (2013). Optimization of Multilevel Ethanol Leaching Process of Porang Flour (Amorphophallus muelleri) Response Surface Using Methodology. International Journal on Advanced Science and Engineering Information Technology, 3(2).

Febrianti, E. P., & Wardani, R. K. (2022). Reduksi Kadar Oksalat dalam Umbi Porang Menggunakan Variasi Konsentrasi, Suhu dan Lama Perendaman dalam Larutan NaCl dan Akuades. Rekayasa, 15(3), 362-367.

Firdaus, R. Z. (2019). Pengaruh penambahan Garam NaCl dan Campuran Mikroorganisme Acetobacter aceti dan Saccharomyces cerevisiae Terhadap Perubahan Kadar Oksalat dan Asam Total pada Fermentasi Kubis (Brassica oleraceae). [SKRIPSI]. Malang: FMIPA . Universitas Brawijaya

Ferdian, M. A., & Perdana, R. G. (2021). Teknologi Pembuatan Tepung Porang Termodifikasi Dengan Variasi Metode Penggilingan Dan Lama Fermentasi. Jurnal Agroindustri, 11(1), 23-31.

Hamdhan, R. Al. (2021). Dampak Usahatani Komoditas Porang Terhadap Kesejahteraan Masyarakat Di Desa Klangon, Kecamatan Saradan, Kabupaten Madiun. Agricore: Jurnal Agribisnis Dan Sosial Ekonomi https://doi.org/10.24198/agricore.v5i2.30614

Handayani, T., Aziz., Y. T., Herlinasari, Depit. (2020). Pembuatan dan Uji Mutu Tepung Umbi Porang (Amorphopallus Oncophyllus Prain) di Kecamatan Ngrayun. Jurnal Medfarm: Farmasi dan kesehatan. 9: 13-21.

Helmi, H., & Eni, K. (2024). The Physical, Chemical, Microbiological, Antibacterial and Prebiotic Characteristics of Fermented Porang Flour with Addition of Bacteria and Yeast. Biosaintifika: Journal of Biology & Biology Education, 15(1).

Helmi, H., Kusmiadi, R., Mahardika, R. G., & Karsiningsih, E. (2024). Diversity of fungi and dynamics of ethanol concentration during fermentation of porang (Amorphophallus oncophyllus). Biodiversitas Journal of Biological Diversity, 25(2).

Hidayah, R. (2016). Budidaya umbi porang secara intensif. Universitas gajah mada.

Hidayah, N., Suhartanto, MR and Santosa, E. (2018) 'Pertumbuhan dan Produksi Iles-iles ( Amorphophallus muelleri Blume) dari Berbagai Teknik Budidaya,Bul. Agrohorti, 6(3), hlm. 405–411. doi: 10.1017/CBO9781107415324.004.

J. Fiedurek, M. Skowronek, A. Gromada. (2011) Selection and adaptation of S. cerevisiae to increased ethanol tolerance and production, Pol. J. Microbiol. 60. 51–58.

Kurniawati, A. D., & Widjanarko, S. B. (2010). Pengaruh Tingkat Pencucian dan Lama Kontak Dengan Etanol Terhadap Sifat Fisik Dan Kimia Tepung Porang (Amorphophallus Oncophyllus). Malang: Universitas Brawijaya.

McLellan MR, Lind LR, Kime RW. (1994). Hue angle determinations and statistical analysis for multiquadrant hunter L, a, b data. J Food Quality 18: 235–240. DOI: 10.1111/j.1745-4557.1995.tb00377. x.

Mardani, A., J. Sumarmono., & T. Setyawardani. (2013). Total Bakteri Asam Laktat Kadar Air, dan Protein Keju Peram Susu Kambing yang Mengandung Probiotik Lactobacillus casei dan Bifidobacterium longum. Jurnal Imliah Peternakan, 1(1):244-253.

Mukhtar, K., Asgher, M., Afghan, S., Hussain, K. dan Zia ul-Hussnain, S. (2010). Comparative study on two commercial strains of S. cerevisiae for optimum ethanol production on industrial scale. Journal of Biomedicine and Biotechnology 2010: 1-5.

Parapouli M, Vasileiadis A, Afendra AS, Hatziloukas E. (2020). S. cerevisiae and its industrial applications. AIMS Microbiol 6: 1-31. DOI: 10.3934/microbiol.2020001.

Pasaribu, G., Hastuti, N., Efiyanti, L., Waluyo, T. K., dan Pari, G., (2019). Optimasi Teknik Pemurnian Glukomanan pada Tepung Porang (Amorphophallus muelleri Blume). Jurnal Penelitian Hasil Hutan. 37(3): 201-208

Pangestika, L. M. W., Lioe, H. N., Adawiyah, D. R., Suliantari, S., Melzer, G., & Weinreich, B. (2021). Penggunaan ekstrak khamir sebagai nutrisi tambahan pada fermentasi moromi kecap kedelaI. Jurnal Teknologi Pertanian, 22(1), 1-12.

Pérez-Gallardo R V, Briones LS, Díaz-Pérez AL, Gutiérrez S, RodríguezZavala JS, Campos-García J. (2013). Reactive oxygen species production induced by ethanol in S. cerevisiae increases because of a dysfunctional mitochondrial iron-sulfur cluster assembly system. FEMS Yeast Res 13: 804-819. DOI: 10.1111/1567- 1364.12090.

Pratama, A., Balia, R. L., & Suryaningsih, L. (2021). Pengaruh Penambahan Yeast (Candida apicola) Pada Sosis Fermentasi Daging Domba Terhadap Kualitas Fisik, Kimia dan Akseptabilitas. Agrointek: Jurnal Teknologi Industri Pertanian, 15(2), 574-582.

Ramadhan, F. S., Rahim, H., & Wardhani, D. H. (2012). Kajian pertumbuhan Lactobacillus casei pada substrat porang (Amorphopallus oncophillus). Jurnal Teknologi Kimia Dan Industri, 1(1), 237-244.

Rodrigues B, Peinado JM, Raposo S, Constantino A, Quintas C, LimaCosta ME. (2015). Kinetic and energetic parameters of carob wastes fermentation by S. cerevisiae: Crabtree effect, ethanol toxicity, and invertase repression. J Microbiol Biotechnol 25: 837- 844. DOI: 10.4014/jmb.1408.08015.

Riesute R, Salomskiene J, Moreno DS, Gustiene S. (2021). Effect of yeasts on food quality and safety and possibilities of their inhibition. Trends Food Sci Technol 108: 1-10. DOI: 10.1016/j.tifs.2020.11.022.

Röling, W, F, -M., Timotius, K, -H., Prasetyo, A, -B., Stouthamer, A, -H., Van Verseveld, H, -W. (1994). Changes in microflora and biochemical composition during the Baceman stage of traditional indonesian kecap (soy sauce) production. Journal of Fermentation and Bioengineering. 77, 62–70. https://doi. org/10.1016/0922-338X(94)90210-0

Sari. I.M., Noverita dan Yulneriwarni. (2008). Pemanfaatan jerami padi dan alang-alang dalam fermentasi etanol menggunakan kapang Trichoderma viride dan khamir S.s cerevisiae. Vis Vitalis.5(2):55-62.

Standar Nasional Indonesia (SNI). (2013). Serpih porang (SNI 7939-2013). Badan Standardisasi Nasional, Jakarta.

Varize CS, Bücker A, Lopes LD, Christofoleti-Furlan RM, Raposo MS, Basso LC, Stambuk BU. (2022). Increasing ethanol tolerance and ethanol production in an industrial fuel ethanol S. cerevisiae strain. Fermentation 8: 1-14. DOI: 10.3390/fermentation8100470.

Wahono, S.K., Damayanti, E., Rosyida, V.T. dan Sadyatu, E.I. (2011). Laju Pertumbuhan S. cerevisiae pada Proses Fermentasi Pembentukan Bioetanol dari Biji Sorgum (Sorghum bicolor L.) Conference: Proceedings of National Seminar on Chemical Engineering and Processes - Diponegoro University, Semarang, Indonesia, ISSN: 1411- 4216

Wardani, A. K., & Pertiwi, F. N. E. (2013). Produksi etanol dari tetes tebu oleh S. Cerevisiae pembentuk flok (Nrrl–Y 265). Agritech, 33(2).

Wardani, R. K., & Handrianto, P. (2019). Pengaruh Perendaman Umbi dan Tepung Porang Dalam Sari Buah Belimbing Wuluh Terhadap Sifat Fisik dan Kadar Kalsium Oksalat. Journal of Pharmacy and Science, 4(2).

Widhiastiti, N. P. U., Darmayanti, L. P. T., & Pratiwi, I. D. P. K. (2022). Pengaruh Lama Fermentasi dengan Lactobacillus plantarum terhadap Karakteristik Fisikokimia dan Fungsional Tepung Biji Durian (Durio zibethinus Murr). Jurnal Ilmu dan Teknologi Pangan (ITEPA), 11(1), 100-111.

Widjanarko, S.B., Megawari, J. (2015). Analisis Metode Kolorimetri dan Gravitasi Pengukuran Kadar Glukomanan Pada Konjak (Amorphopallus konjac). Jurnal Pangan dan Agroindustri. Vol 3:1

Widjanarko, S. B., & Mawarni R. (2015). Penggilingan Metode Ball Mill dengan Pemurnian Kimia Terhadap Penurunan Oksalat Tepung Porang. Jurnal Pangan dan Agroindustri, 3(2):571-581.

Wulandari, F., Nazaruddin, N., & Amaro, M. (2021). Pengaruh jenis bakteri asam laktat dan lama fermentasi terhadap mutu fisik, kimia, organoleptik dan mikrobiologi tepung mocaf. Prosiding SAINTEK, 3, 169-181.

Wulandari, A. (2018). Pengaruh Lama Waktu Fermentasi Kombucha Teh Hijau Daun Jati ( Tectona grandis) Terhadap Kadar Tanin Total dan Asam Total Tertitrasi (TAT). Program Studi Pendidikan Biologi Jurusan Pendidikan Matematika Dan Ilmu Pengetahuan Alam Fakultas Keguruan Dan Ilmu Pendidikan Universitas Sanata Dharma Yogyakarta 2018, 202.

Zarubica, A.R., Miljkovic, M. N., Purenovic, M. M., & Tomic, V. B. (2005).Color Parameters, Whiteness Indices and Physical Features Of Making Paints For Horizontal Signalization. Facta Universitatis (Physics, Chemistry, and Technology), Serbia, Montenegro. 3:205-216.

Downloads

Published

2026-01-26

How to Cite

Wati, I., Helmi, H., & Lingga, R. (2026). The Effect of Addition Various Concentrations of Yeast (Saccharomyces cerevisiae) on the Quality of Porang Tubers (Amorphophallus sp.). JURNAL BIOS LOGOS, 15(3), 324–335. https://doi.org/10.35799/jbl.v15i3.61693

Similar Articles

1 2 > >> 

You may also start an advanced similarity search for this article.