KARAKTERISASI PRODUK GEN PENGKODE Chaperone Bacillus sp. RP1

Authors

  • Maureen Kumaunang Universitas Sam Ratulangi
  • Yohanis Mandik Universitas Cendrawasih, Jayapura

DOI:

https://doi.org/10.35799/cp.3.1.2010.75

Abstract

Molecular chaperone is the group of protein that assist in the correct folding or assembly of other proteins invivo. The objective of this research was to characterize the product of gene encoding chaperone from Bacillussp. RP1 by using in-silico analysis. Analysis of 1053 bp from DNA fragment isolated by PCR showed that it has97% similarity with putative chaperone of Geobacillus kaustophilus (BA000043.1) and 86% similarity with DnaKoperon of B. stearothermophilus (X90709.1). Structure prediction of DnaJ Bacillus sp. RP1 showed that it hassimilar J-domain with that of DnaJ Escherichia coli but cys-rich domain is different.

Author Biographies

Maureen Kumaunang, Universitas Sam Ratulangi

Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Sam Ratulangi, Manado

Yohanis Mandik, Universitas Cendrawasih, Jayapura

Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Cendrawasih, Jayapura

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Published

2019-12-13

How to Cite

Kumaunang, M., & Mandik, Y. (2019). KARAKTERISASI PRODUK GEN PENGKODE Chaperone Bacillus sp. RP1. CHEMISTRY PROGRESS, 3(1), 52–56. https://doi.org/10.35799/cp.3.1.2010.75

Issue

Vol. 3 No. 1 (2010)

Section

Articles