Antagonist Test of Gliocladium sp. against Fusarium sp. Cause of Wilt Disease in Shallot Plants In Vitro
DOI:
https://doi.org/10.35799/jbl.v15i2.62836Keywords:
antagonist, Fusarium sp., Gliocladium sp., dual cultureAbstract
This study aims to analyze the morphological characteristics of the fungus Gliocladium sp., measure its inhibition against the growth of the fungus Fusarium sp. causing wilt disease in shallot plants (Allium ascalonicum L), and examine its antagonistic mechanism in vitro. This research method includes exploration and sampling, sterilization of tools and materials, making PDA media, isolation, purification, and identification of morphological characteristics and measurement of antagonistic fungal inhibition. The antagonist test uses the dual culture method, and data analysis with a completely randomized design (CRD) and ANOVA test followed by the Honestly Significant Difference (HSD) Test at the 5% significance level. The results showed that Gliocladium sp. was able to inhibit the growth of Fusarium sp. with a percentage inhibition of 25.63% and spore viability of 66.52%. Antagonistic mechanisms observed include competition for space and nutrients, antibiosis, lysis, and mycoparasitism.
References
Abo-Elyousr, K. A., Abdel-Hafez, S. I., & Abdel-Rahim, I. R. (2014). Isolation of Trichoderma and evaluation of their antagonistic potential against Alternaria porri. Journal of Phytopathology, 162(9), 567-574.
Gupta, V., Sharma, R., & Kumar, V. (2018). Efficacy of Gliocladium sp. in controlling Fusarium wilt of rice under field conditions. International Journal of Pest Management, 64(1), 65-74.
Maqbool, M., Arif, M., & Ali, S. (2017). Effects of Gliocladium sp. on root rot and growth of garlic. Journal of Plant Diseases and Protection, 124(3), 161-170.
Prakoso, E. B., Wiyatingsih, S., & Nirwanto, H. (2016). Uji ketahanan berbagai kultivar bawang merah (Allium ascalonicum L.) terhadap infeksi penyakit moler (Fusarium oxysporum f.sp. cepae). Berkala Ilmiah Agroteknologi- PLUMULA, 5(1),10-20.
Rizal, S. (2017). Uji antagonis Gliocladium sp. dalam menghambat pertumbuhan cendawan penyebab penyakit busuk antraknosa (Colletotrichum capsici). J. Sainmatika, 14 (2), 100-106
Rosyidah, N. (2019). Analisis Penetapan Harga Jual Bawang Merah Dalam Perspektif Ekonomi Islam di Desa Sengon Kec.Tanjung Kab. Brebes. [Skripsi, Semarang: Universitas Islam Negeri Walisongo]
Sharma, A., Sharma, S., & Dalal, R. (2019). Gliocladium: A mycoparasite for biocontrol of plant pathogens—A review. Agricultural Reviews, 40(2), 107-112.
Singh, R., Singh, M., & Saini, V. (2019). Efficacy of Gliocladium sp. in controlling maize diseases and improving crop yield. Journal of Agricultural Science, 157(1), 22-31.
Soesanto, L. (2008). Pengantar Pengendalian Hayati Penyakit Tanaman. Rajawali Press. Jakarta
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Dea Sarayar, Stella Deiby Umboh, Parluhutan Siahaan
This work is licensed under a Creative Commons Attribution 4.0 International License.
