Development of Commercial Sterility Detection Method Based on Chromogenic Media Agar in UHT Coconut Cream Liquid Products
DOI:
https://doi.org/10.35799/jbl.v15i3.64802Keywords:
commercial sterility, chromogenics agar, coconut cream liquid, Ultra High TemperatureAbstract
Commercial sterility analysis is a crucial step in quality control of Ultra High Temperature (UHT) products such as coconut cream liquid products produced by PT Sasa Inti Minahasa Selatan, to ensure the safety and stability of shelf life. Stability incubation testing takes a long time, which is 7-14 days. Development of commercial sterility methods using Chromogenic Agar selective media is needed to cut the incubation time that is too long. Agar Chromogenic Media is tested for its ability to detect common contaminants in UHT coconut cream products, in particular thermophilic and mesophilic microorganisms that cause spoilage, with a short incubation time. UHT coconut cream samples are prepared by artificial and natural inoculation. Validation was carried out by comparing the results on standard media based on sensitivity, specificity and Time-to-result (TTR) parameters. The results showed a real difference between NA/TGEA media and chromogenic media so that the reading of bacterial colonies growing on chromogenic media was clearer and easier to read due to the change in color and short incubation time where the bacteria causing decay had grown on day 5 with a reading of results that took only 24 hours. Based on the validation that has been carried out, chromogenic selective media can accelerate product release time and improve quality control efficiency in the UHT coconut cream industry.
References
AOAC International. (2016). AOAC Official Method 972.44: Microbial Plate Count (Bacterial) Chapter 17. Official Methods of Analysis of AOAC International (20th ed.). AOAC International.
Fuchs, E., Raab, C., Brugger, K., Schulz, M., Wagner, M., Stessl, B. (2022). Performance Testing of Bacillus cereus Chromogenic Agar Media for Improved Detection in Cream and Other Food Samples. Journal Foods 11 (288) 1-16.
Food and Drug Administration. (2020). Bacteriological Analytical Manual (BAM) Chapter 23: Microbiological Methods for Cosmetics. Silver Spring: FDA.
Gazin, M., Paasch, F., Goossens, H., Malhotra-Kumar, S. (2008). Extended-Spectrum Beta-Lactamase Detection with Different Panels for Automated Susceptibility Testing and with a Chromogenic Medium. Journal of Clinical Microbiology, 46(11), 3721–3727.
Haryadi, P (2024) Materi 1. Pendahuluan, Pemahaman, Persyaratan & Regulasi Proses Termal. Proses Termal untuk Industri Pangan, Batch 22.
Manafi, M. (1996). Fluorogenic and Chromogenic enzyme substrates in culture media and identification test. Int J Food Microbiol, 31(1-3): 45-58.
Merck KGaA. (2018). Bacillus ChromoSelect Agar: NutriSelect® Plus (dehydrated culture medium) 92325. Darmstadt: MilliporeSigma 1-3.
Oxoid. 2006. Manual Oxoid. Edisi 9. Oxoid Limited: Bandung.
Putri, G., Senjawati, M., Erlinanto, Y. (2020) Analisis Penyebab Cacat Produk Santan Kemasan dalam Penetapan Critical Control Point dengan Pendekatan HACCP di PT. X.Inventory: Industrial Vocational E-Journal on Agroindustry, 5(02): 64-71
Tetrapak Method (2009). Procedure & Guideline: Quality Control routines in a Dairy Industry. QAM-588017-0101: (53-57).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Ester Ruth Youla Tangkau, Chindy Achika Rori, Yoel William Prasetyo Kamto, Brayen Mangangantung, Agustina Monalisa Tangapo
This work is licensed under a Creative Commons Attribution 4.0 International License.
