AKTIVITAS ANTIBAKTERI EKSTRAK AIR REBUSAN DAUN MANGROVE SEGAR Sonneratia alba DI DESA WORI KABUPATEN MINAHASA UTARA
DOI:
https://doi.org/10.35800/mthp.7.2.2019.23612Keywords:
Antibacterial, infusion, Sonneratia alba, antibakteri, infusaAbstract
The purpose of this study was to determine the antibacterial activity in the extracts of Sonneratia alba mangrove leaf inhibiting Staphylococcus aureus and Escherichia coli bacteria. The infusion extraction method was used to extract fresh leaves. Fresh leaves was boiled for 40 and 50 minutes then was boiled again with small fire continued with dried in an oven with a temperature of 75–80°C. The ananlysis was conducted to  an extract  of 5% and 10%, positive control (chloramphenicol), and negative control (aquades). The disc diffusion Kirby and Bauer method which has been modified was performed in these samples. The results showed that antibacterial activity from extracts of fresh mangrove leaf  was shown against both tested bacteria and explained the broad spectrum antibacterial compounds.
Keyword: Sonneratia alba, antibacterial, infusion.
Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antibakteri yang terdapat pada ekstrak air rebusan daun mangrove Sonneratia alba dalam menghambat bakteri Staphylococcus aureus dan Escherichia coli. Metode ekstraksi infusa digunakan untuk mengekstrak daun mangrove segar. Caranya yaitu daun direbus selama 40 dan 50 menit setelah itu direbus kembali dengan air api kecil kemudian dikeringkan dalam oven dengan suhu 75–80°C. Pengujian dilakukan pada konsentrasi ekstrak 5% dan 10%, kontrol positif (kloramfenikol), dan kontrol negatif (akuades). Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar (disc diffusion Kirby and Bauer) yang telah dimodifikasi. Hasil yang diperoleh yaitu ekstrak air rebusan daun mangrove segar memiliki sifat antibakteri terhadap kedua bakteri uji sehingga senyawa antibakteri dari ekstrak air rebusan daun mangrove dikategorikan berspektrum luas.
Kata kunci: Sonneratia alba, antibakteri, infusa.
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